lID Applications in Oncology
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I I I pg PRA ~ F.
5.5 pg PRAME
1.1 PI! PRAME
" 0.55 PI! PR AM E
5 0. 11 pg PRAME
6 2.5 pg PRAME
(K51i2 cell line)
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Fig.2a,b. Quantification of the antigen PRAME by serial dilution of a PRAME-PCR product. The
amplification curves of different amounts of PRAME cDNA (a) and the melting curves of the
standard curves (b). PCR of 50 ng K562 cDNA showed 2.5 pg of PRAME amplification starting
product