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Rapid cycle real-time PCR-methods and applications : genetics and oncology
- Date_TXT
- Berlin : Springer , 2002
- Cote
- 576.83 DIE
- Auteur
- Dietmaier, Wolfgang, Sivasubramanian, Natarajan, Wittwer, Carl
- Type de document
- Livre
Description :
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green allow product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms. The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and analysis in the fields of Genetics and Oncology. Molecular analysis has never been easier.