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Quantitative Measurement of the mRNA Expression of the 1\unor-Associated Antigen PRAME m
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Cycle Number
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70
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74
76
78
80
82
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T efT1lefatl,le ('C)
()
ce ll~ of healthy olullIeers
I x 11)' K5(J2 cell~
9.9x 10" cells ofheallhy volumccl>
IxlO" KS62 <..,11,
' 1.1)<).10' cells of healthy VOI\1nlee",
I. 10' K562 cell'
4 9.999.10" cells of heallhy volunleers
I x 10" K562 cells
5 I x III' cclls of hCIllthy VOIU"Iccrs
I,m' K.'\1>2 cc ll s
6 I x I 0' cd ls of hcaltl,y v"lu",,,,,,,,
I x I 0' KS62 ,,,II,
7 I 10' cells ()f healthy volunteers
I. 10' KS(,2 cells
S I x 10' "clb "f healthy voIUnlc'CI1i
Ix 10" KS621"lb
Fig.la,b. Sensitivity of the PRAME LightCycler RT-PCR detecting leukemia K562 cells (PRAME
positive) mixed with normal mononuclear cells (PRAME negative). a Amplification curves,
b melting curve analysis. With the LightCycler RT-PCR a ratio of one leukemia cell (positive for
PRAME) in lxlOs normal mononuclear cells (negative for PRAME) could be detected