Quantitative Analysis of AMLI-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR ITiJiJ
taken twice a week. AMLl-ETO was normalized to the housekeeping genes
G6PDH and cABL. The relative quantification of AMLl-ETO/G6PDH and AMLlETO/cABL at each point of analysis during induction therapy revealed virtually
identical results. Thus G6PDH as well as cABL are appropriate reference genes for
AMLl-ETO quantification. This confirms data described previously for quantitative analysis of BCR-ABL fusion transcripts in CML [10).
Quantification of AMLl-ETO levels using real-time RT-PCR during the course of
therapy of a patient demonstrates that real-time PCR has the potential to diagnose molecular relapse before it is detectable by cytogenetics or cytomorphology
(Fig. 6).
Conclusions
Quantitative monitoring of AMLl-ETO fusion transcript levels in t(8;2l) positive
AML using real-time PCR offers several advantages. Compared to competitive
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Fig.3b. Standard curve of AMLl-ETO (a) and G6PDH (b) using serial dilutions of Kasumi 1 cells
(1:10 - 1:lO000) plus quantitative analysis of a patient
Quantitative RealTime peR has the
Potential to Predict
Hematological
Relapse Before Clinical Manifestation