LightCyder PCR for the Polymorphisms - 308 and - 238 in the TNF Alpha Gene n
LightCycler settings for TNF-a G-30BA and G-23BA:
Experimental
Protocols
Denaturation
Cydes
Type
None
Segment
Number
Amplification
Cycles
Type
Segment
Number
I
2
3
Melting
Cycles
Type
Segment
Number
I
2
3
Cooling
Cycles
Temperature
Target [0C)
95
70
None
Temperature
Ta.rget [0C)
95
50
72
Melting Curves
Temperature
Ta.rget [0C)
95
45
S5
Type
None
Segment
Number
I
Temperature
Target [0C)
40
Fluorescence sCllings
LED power
FI Gain
CALIB
F2 Gain
15
F3 Gain
30
Hold Time
[s)
120
Hold Time
[s)
0
10
10
Hold Time
[s)
0
90
0
Hold Time
(s)
30
Slope
[OC/s)
20
Slope
[OCls)
20
20
5
Slope
[OCls)
20
20
0.1
Slope
(OCls)
20
Acquisition
Mode
None
Acquisition
Mode
None
Single
None
Acquisition
Mode
None
None
Continuous
Acquisition
Mode
None
Fluorescence
Display Mode
F2Il (G-308A)
F3f1 (G-238A)
Fluorescence
Display Mode
F211 (G-30SA)
F3/1 (G-23SA)
Fluorescence
Display Mode
F2/1 (G-30SA)
F3/1 (G-238A)
Fluorescence
Display Mode
F2/1 (G-30SA)
F3/1 (G-23SA)