Iti!l1I Applications in Genetics
LightCycler settings for TNF-/3 A329G:
Denaturation
Cycles
Type
None
Segment
Temperature
Number
Target 1°C]
1
95
Amplification
Cycles
45
Type
None
Segment
Temperature
Number
Target roC]
1
95
2
60
3
72
Melting
Cycles
Type
Melting Curves
Segment
Temperature
Number
Target roC]
95
2
45
3
80
Cooling
Cycles
Type
None
Segment
Temperature
Number
Target (O C]
I
40
fluorescence settings
LED power
PI Gain
F2 Gain
F3 Gain
CALIB
7
10
Hold Time
Is]
0
Hold Time
[s]
0
10
13
Hold Time
Is]
0
30
0
Hold Time
[5]
30
Slope
Acquisition
Fluorescence
[OC/s]
Mode
Display Mode
0
None
F2/l (A329G)
Slope
Acquisition
Fluorescence
[O C/sl
Mode
Display Mode
20
None
F2I1 (A329G)
20
Single
5
None
Slope
Acquisition
Fluorescence
IO C/s]
Mode
Display Mode
20
None
F2/1 (A329G)
20
None
0.1
Continuous
Slope
Acquisition
Fluorescence
[O C/s ]
Mode
Display Mode
20
None
P2I l (A329G)