Processes 2018, 6,38
Figure A2. (a) Lysis of DNA standard did not significantly influence Hoechst fluorescent response.
Error bars represent three technical replicates of standard solutions. Neither slopes nor intercepts of the
two curves are significantly different (p > 0.05, T-tests). (b) Washing samples with cell wash solution
resulted in significantly lower DNA recovery from E. coli samples (p < 0.05, T-test). Freezing samples
did not have a significant impact on DNA recovery (p > 0.05, T-test).
Figure A3. Autoclaving samples prior to chloroform–methanol lipid extraction did not significantly
enhance lipid recovery. Solid columns represent unautoclaved samples and striped columns represent
autoclaved samples. Lipid recovery was not significantly affected by autoclaving for A. acidocaldarius
(p > 0.05, T-test), whereas lipid recovery was slightly lower for autoclaved E. coli samples (0.05 < p < 0.01,
T-test). n = 9 and 3 for unautoclaved and autoclaved E. coli samples, respectively, and n = 6 and 3 for
unautoclaved and autoclaved A. acidocaldarius, respectively.
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Figure A2. (a) Lysis of DNA standard did not significantly influence Hoechst fluorescent response.
Error bars represent three technical replicates of standard solutions. Neither slopes nor intercepts of the
two curves are significantly different (p > 0.05, T-tests). (b) Washing samples with cell wash solution
resulted in significantly lower DNA recovery from E. coli samples (p < 0.05, T-test). Freezing samples
did not have a significant impact on DNA recovery (p > 0.05, T-test).
Figure A3. Autoclaving samples prior to chloroform–methanol lipid extraction did not significantly
enhance lipid recovery. Solid columns represent unautoclaved samples and striped columns represent
autoclaved samples. Lipid recovery was not significantly affected by autoclaving for A. acidocaldarius
(p > 0.05, T-test), whereas lipid recovery was slightly lower for autoclaved E. coli samples (0.05 < p < 0.01,
T-test). n = 9 and 3 for unautoclaved and autoclaved E. coli samples, respectively, and n = 6 and 3 for
unautoclaved and autoclaved A. acidocaldarius, respectively.
175