Biomarkers
G. mikimotoi. These data, recalculated from a previous experiment (Parnsh et al., 1994), show rhat most lipids occur in a glycolipid fraction
which includes also pigments and monoacylglycerols. The cell quota
of this fraction increased during the culture until it stabilized near the
maximum of cell density at a value of 49 ± 4.6% (n = 4) of total lipids.
Figure 1b shows histograms representing the relative proportions in the
lipid extract of phospholipids (PL) and glycolipids (GL) after fractionation
into its component classes MGDG, DGDG, SQDG.
The distribution among the polar lipids (PL + GL) appeared specific to
each strain while neutral lipids (100% polar lipids) represented a
relatively constant proportion ol the total lipids in the three strains
(36.5 ± 1.9%, n = 3).
Figure 2 illustrates the distribution of the major unsaturated fatty acids
in the acyl compounds of cellular extracts. Octadecatetraenoic acid
(18:4 (n-3) and eicosapentaenoic acid (20:5 (n-3) predominated in
H. akashiwo while octadecapentaenoic acid (18:5 (n-3) and docosahexaenoic acid (22:6 (n-3) were the major PUFAs in both Gymnodinium
strains with a proportion accounting for 14-29% of total fatty acids.
Figure 2
The major (< 4% in at least
one species) unsaturated
fatty acids in G. mikimotoi,
G. coru and H. akashiwo
cells sampled at late-log
phase.
The distribution of the major PUFAs in the two galactolipid classes
(MGDG and DGDG) of Gymnodinium strains is presented in figure 3.
Hexadecatetraenoic acid (16:4 (n-3) was detected in G. mikimotoi in
higher proportions than 18:4 (n-3) and vice versa for G. corii galactolipids. The acid 18:5 (n-3) was high in the two species but more prominent
in G. corii with 67 and 52% of total fatty acids respectively.
69
G. mikimotoi. These data, recalculated from a previous experiment (Parnsh et al., 1994), show rhat most lipids occur in a glycolipid fraction
which includes also pigments and monoacylglycerols. The cell quota
of this fraction increased during the culture until it stabilized near the
maximum of cell density at a value of 49 ± 4.6% (n = 4) of total lipids.
Figure 1b shows histograms representing the relative proportions in the
lipid extract of phospholipids (PL) and glycolipids (GL) after fractionation
into its component classes MGDG, DGDG, SQDG.
The distribution among the polar lipids (PL + GL) appeared specific to
each strain while neutral lipids (100% polar lipids) represented a
relatively constant proportion ol the total lipids in the three strains
(36.5 ± 1.9%, n = 3).
Figure 2 illustrates the distribution of the major unsaturated fatty acids
in the acyl compounds of cellular extracts. Octadecatetraenoic acid
(18:4 (n-3) and eicosapentaenoic acid (20:5 (n-3) predominated in
H. akashiwo while octadecapentaenoic acid (18:5 (n-3) and docosahexaenoic acid (22:6 (n-3) were the major PUFAs in both Gymnodinium
strains with a proportion accounting for 14-29% of total fatty acids.
Figure 2
The major (< 4% in at least
one species) unsaturated
fatty acids in G. mikimotoi,
G. coru and H. akashiwo
cells sampled at late-log
phase.
The distribution of the major PUFAs in the two galactolipid classes
(MGDG and DGDG) of Gymnodinium strains is presented in figure 3.
Hexadecatetraenoic acid (16:4 (n-3) was detected in G. mikimotoi in
higher proportions than 18:4 (n-3) and vice versa for G. corii galactolipids. The acid 18:5 (n-3) was high in the two species but more prominent
in G. corii with 67 and 52% of total fatty acids respectively.
69