255
such techniques to microbial ecology and oceanography will lead to a better understanding of
predator-prey dynamics, the spatial and temporal variability of species, and syntrophic
microbial interactions, as they occur in mixed populations.
ACKNOWLEDGEMENTS
I would like to thank to Dr. Norman Pace for advice and support, Dr. Thomas Schmidt for collaborative effort
in the analysis of Central North Pacific bacterioplankton, and Dr. David Karl for hospitality and ship time at the
Aloha Station. This work was funded in part by Office of Naval Research Young Investigator Award
NOO014-90-J-1917 to EFD. This is Woods Hole contribution number 7564.
REFERENCES
Amann RI, Krumholz L, Stahl DA (1990a) Fluorescent oligonucleotide probing of whole cells
for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol
172: 762-770
Amann RI, Binder BB, Olson RJ, Chisholm SW, Devereux, Stahl DA (1990b) Combination
of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed
microbial populations. Appl Environ Microbiol 56: 1919-1925
Baumann P, Baumann L, Woolkalis MJ, Bang SS (1983) Evolutionary relationships in Vibrio
and Photobacterium: a basis for a natural classification. Ann Rev Microbiol
37:369-398
DeLong EF, Wickham GS, Pace NR (1989a) Phylogenetic stains: Ribosomal RNA-based
probes for the identification of single cells. Science 243: 1360-1363.
DeLong EF, Schmidt TM, Pace NR (1989b) Analysis of single cells and oligotrophic
picoplankton populations using 16S rRNA sequences. In: Hattori T, Ishida Y,
Maruyama Y, MoritaRYand A.Uchida, (eds) Recent Advances in Microbial Ecology.
Jap Sci Soc Press, Tokyo, p 697
DeLong EF, Shah J (1990) Fluorescent ribosomal RNA probes for clinical application: a
research review. Diagnos Clinc Test 28:41-44.
Ducklow HW (1983) Production and fate of bacteria in the oceans. Bioscience 33:494-501
Eckert KA, Kunkel TA (1990) High fidelity synthesis by the Thermus aquaticus DNA
polymerase. Nuc Acid Res 18:3739-3744
Ennis PD, Zemmour J, Salter RD, Parham P (1990) Rapid cloning of HLA-A,B cDNA by
using the polymerase chain reaction: frequency of errors produced in amplification.
Proc Natl Acad Sci 87:2833-2837
Felsenstein J (1988) Phylogenies from molecular sequences: inference and reliability. Annu
Rev Genet 22:521-565
Felsenstein J (1982) Numerical methods for inferring evolutionary trees. Quart. Rev. BioI.
57:379-404
Fenchel T (1982) Ecology of heterotrophic microflagellates: quantitative occurrence and
importance as consumers of bacteria. Mar Ecol Prog Ser 9:35-42
Ferguson RL, Buckley EN, Palumbo AL (1984) Response of marine bacterioplankton to
differential filtration and confinement. Appl Environ Microbiol 47:49-55
such techniques to microbial ecology and oceanography will lead to a better understanding of
predator-prey dynamics, the spatial and temporal variability of species, and syntrophic
microbial interactions, as they occur in mixed populations.
ACKNOWLEDGEMENTS
I would like to thank to Dr. Norman Pace for advice and support, Dr. Thomas Schmidt for collaborative effort
in the analysis of Central North Pacific bacterioplankton, and Dr. David Karl for hospitality and ship time at the
Aloha Station. This work was funded in part by Office of Naval Research Young Investigator Award
NOO014-90-J-1917 to EFD. This is Woods Hole contribution number 7564.
REFERENCES
Amann RI, Krumholz L, Stahl DA (1990a) Fluorescent oligonucleotide probing of whole cells
for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol
172: 762-770
Amann RI, Binder BB, Olson RJ, Chisholm SW, Devereux, Stahl DA (1990b) Combination
of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed
microbial populations. Appl Environ Microbiol 56: 1919-1925
Baumann P, Baumann L, Woolkalis MJ, Bang SS (1983) Evolutionary relationships in Vibrio
and Photobacterium: a basis for a natural classification. Ann Rev Microbiol
37:369-398
DeLong EF, Wickham GS, Pace NR (1989a) Phylogenetic stains: Ribosomal RNA-based
probes for the identification of single cells. Science 243: 1360-1363.
DeLong EF, Schmidt TM, Pace NR (1989b) Analysis of single cells and oligotrophic
picoplankton populations using 16S rRNA sequences. In: Hattori T, Ishida Y,
Maruyama Y, MoritaRYand A.Uchida, (eds) Recent Advances in Microbial Ecology.
Jap Sci Soc Press, Tokyo, p 697
DeLong EF, Shah J (1990) Fluorescent ribosomal RNA probes for clinical application: a
research review. Diagnos Clinc Test 28:41-44.
Ducklow HW (1983) Production and fate of bacteria in the oceans. Bioscience 33:494-501
Eckert KA, Kunkel TA (1990) High fidelity synthesis by the Thermus aquaticus DNA
polymerase. Nuc Acid Res 18:3739-3744
Ennis PD, Zemmour J, Salter RD, Parham P (1990) Rapid cloning of HLA-A,B cDNA by
using the polymerase chain reaction: frequency of errors produced in amplification.
Proc Natl Acad Sci 87:2833-2837
Felsenstein J (1988) Phylogenies from molecular sequences: inference and reliability. Annu
Rev Genet 22:521-565
Felsenstein J (1982) Numerical methods for inferring evolutionary trees. Quart. Rev. BioI.
57:379-404
Fenchel T (1982) Ecology of heterotrophic microflagellates: quantitative occurrence and
importance as consumers of bacteria. Mar Ecol Prog Ser 9:35-42
Ferguson RL, Buckley EN, Palumbo AL (1984) Response of marine bacterioplankton to
differential filtration and confinement. Appl Environ Microbiol 47:49-55