117
7 Aquaculture to Restocking
Research on providing adequate food and nutrition to grow healthy paralarvae
and juvenile was also performed from the beginning of the SISFFA and JASFA projects. In 2001, JASFA achieved the production of settling paralarvae at a high survival rate through feeding with the frozen Pacific sand eel Ammodytes personatus
and large-sized nauplii of Artemia tibetiana (Shiraki 1997; Okumura et al. 2005).
7.2.2.2 Sepioteuthis lessoniana
Choe and Oshima (1963) reared S� lessoniana from hatching to the age of 45 days
fed on Neomysis japonica. Later, there were several studies to rear this species for
the purpose of aquaculture (Choe 1966; Inoha and Sezoko 1967; Tsuchiya 1982;
Segawa 1987). SISFFA began incubating wild-collected egg masses of this species
for the release of hatchlings in 1963 and reared them to juveniles of 70 mm TL at the
age of 2 months in 1964 (JASFA 2003). In 1982, JASFA restarted experiments to
develop the technology for seed production to restock S� lessoniana in Goto Island,
Nagasaki Prefecture (Fig. 7.1), because of the declining catches of the species, but
this activity ended in 1992 (JASFA 2003). In the first year, eggs were collected from
live females spawning in captivity and from an artificial substrate in the wild. The
artificial substrate was made from tree branches immersed in the spawning ground
as an artificial spawning bed. The collected eggs were then incubated in a tank and
hatchlings were produced. One of the purposes of this project was to develop effective artificial spawning beds for the species. Ueta et al. (1995) developed several
types of effective man-made spawning beds for S� lessoniana.
7.2.2.3 Heterololigo bleekeri
Matsumoto (1976) maintained adults of this species in tanks for 3 weeks. JASFA
addressed the development of technology for spawning and hatching of this species
for use in restocking between 1985 and 1993 in Miyazu which faces Wakasa Bay
in the Kyoto Prefecture (JASFA 2003). In 1985, JASFA reared hatchlings fed on
live zooplankton, Artemia and hatchlings of scorpion fish and produced 134 squid
(6–7 mm in mantle length (ML), with a 0.35 % survival rate from hatchlings) at 40
days after hatching. In 1986, 81,000 eggs were collected from an artificial spawning bed experiment in the sea (JASFA 2003). This project accomplished certain
results in developing adequate spawning beds and ended because of the difficulties
encountered for mass culturing of this species.
7.2.2.4 Sepia latimanus
Inoha (1971) observed that S� latimanus laid eggs and attached them to corals of
the genus Millepora in Okinawa and they were easy to rear in the aquarium. JASFA
has conducted research and development of the technology for seed production of
7 Aquaculture to Restocking
Research on providing adequate food and nutrition to grow healthy paralarvae
and juvenile was also performed from the beginning of the SISFFA and JASFA projects. In 2001, JASFA achieved the production of settling paralarvae at a high survival rate through feeding with the frozen Pacific sand eel Ammodytes personatus
and large-sized nauplii of Artemia tibetiana (Shiraki 1997; Okumura et al. 2005).
7.2.2.2 Sepioteuthis lessoniana
Choe and Oshima (1963) reared S� lessoniana from hatching to the age of 45 days
fed on Neomysis japonica. Later, there were several studies to rear this species for
the purpose of aquaculture (Choe 1966; Inoha and Sezoko 1967; Tsuchiya 1982;
Segawa 1987). SISFFA began incubating wild-collected egg masses of this species
for the release of hatchlings in 1963 and reared them to juveniles of 70 mm TL at the
age of 2 months in 1964 (JASFA 2003). In 1982, JASFA restarted experiments to
develop the technology for seed production to restock S� lessoniana in Goto Island,
Nagasaki Prefecture (Fig. 7.1), because of the declining catches of the species, but
this activity ended in 1992 (JASFA 2003). In the first year, eggs were collected from
live females spawning in captivity and from an artificial substrate in the wild. The
artificial substrate was made from tree branches immersed in the spawning ground
as an artificial spawning bed. The collected eggs were then incubated in a tank and
hatchlings were produced. One of the purposes of this project was to develop effective artificial spawning beds for the species. Ueta et al. (1995) developed several
types of effective man-made spawning beds for S� lessoniana.
7.2.2.3 Heterololigo bleekeri
Matsumoto (1976) maintained adults of this species in tanks for 3 weeks. JASFA
addressed the development of technology for spawning and hatching of this species
for use in restocking between 1985 and 1993 in Miyazu which faces Wakasa Bay
in the Kyoto Prefecture (JASFA 2003). In 1985, JASFA reared hatchlings fed on
live zooplankton, Artemia and hatchlings of scorpion fish and produced 134 squid
(6–7 mm in mantle length (ML), with a 0.35 % survival rate from hatchlings) at 40
days after hatching. In 1986, 81,000 eggs were collected from an artificial spawning bed experiment in the sea (JASFA 2003). This project accomplished certain
results in developing adequate spawning beds and ended because of the difficulties
encountered for mass culturing of this species.
7.2.2.4 Sepia latimanus
Inoha (1971) observed that S� latimanus laid eggs and attached them to corals of
the genus Millepora in Okinawa and they were easy to rear in the aquarium. JASFA
has conducted research and development of the technology for seed production of