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study the effect of touch on tactile refl exes. Refl ex suppression was later applied to
study the sensitivity of the mammalian auditory system by Russo ( 1979 ).
Conditioning paradigms in combination with the suppression of ventilation and cardiac refl exes have also been successfully used in fi shes to determine auditory sensitivity. Recently the suppression of the ASR using a prepulse inhibition (PPI)
paradigm was shown to yield lower auditory thresholds than the ASR assay alone
(Bhandiwad et al. 2013 ; Fig. 6 ).
Fig. 8 Ontogeny of the startle response for larval zebrafi sh ( Danio rerio ; N = 24; left ) and larval
sticklebacks ( Gasterosteus aculeatus ; N = 11; right ). The probability of observing a startle response
for a cohort of fi sh was tested using a repeated measures design. Fish were tested daily after hatching for the presence of the ASR to broadband acoustic stimuli ( top ) and pure tone stimuli of 45 Hz
( middle ), and 90 Hz ( bottom ). Zebrafi sh had a rapid onset of ASR at ~5 dpf. However, sticklebacks
did not show the ASR until 8–13 dpf with frequency-specifi c differences in ASR onset
Revisiting Psychoacoustic Methods for the Assessment of Fish Hearing
study the effect of touch on tactile refl exes. Refl ex suppression was later applied to
study the sensitivity of the mammalian auditory system by Russo ( 1979 ).
Conditioning paradigms in combination with the suppression of ventilation and cardiac refl exes have also been successfully used in fi shes to determine auditory sensitivity. Recently the suppression of the ASR using a prepulse inhibition (PPI)
paradigm was shown to yield lower auditory thresholds than the ASR assay alone
(Bhandiwad et al. 2013 ; Fig. 6 ).
Fig. 8 Ontogeny of the startle response for larval zebrafi sh ( Danio rerio ; N = 24; left ) and larval
sticklebacks ( Gasterosteus aculeatus ; N = 11; right ). The probability of observing a startle response
for a cohort of fi sh was tested using a repeated measures design. Fish were tested daily after hatching for the presence of the ASR to broadband acoustic stimuli ( top ) and pure tone stimuli of 45 Hz
( middle ), and 90 Hz ( bottom ). Zebrafi sh had a rapid onset of ASR at ~5 dpf. However, sticklebacks
did not show the ASR until 8–13 dpf with frequency-specifi c differences in ASR onset
Revisiting Psychoacoustic Methods for the Assessment of Fish Hearing