480
UWE R. MULLER et al.
-
CMYC
..
o 40 r---~-------.;:;
. .
ll::
~ 30
u
~
~ 20 1--------------~
MCL1
•
.,............ HLl ...... n'-!
~
,~...
POUSFh
•
~ 0 -!----- ~~I
10
100
1000
10000
Genes sorted by net CyS signal intensity
about 3000 genes
..... -
-
,g 40
. .
ll::
~30
~
M
~20
"C
~ 10
.. E 0
~ 10
100
1000
Genes sorted by net CyS signal intensity
about 1700 genes
10000
Fig. 2. Gene copy number analysis of tumor cell line Colo320 with cDNA arrays. The top
panel shows all -1700 genes on the cancer cDNA array sorted by CYS signal intensity (reference DNA). The amplified onco-genes cMYC, ABCDI, MCLl, and POUSFI are marked.
The bottom panel shows a self/self hybridization with normal DNA for comparison.
:1") \,
•
/
CYP2~
- ERBB2 --..
-
• • •
• • • •
Fig. 3. Detection of DNA copy-number
changes in tumor cell line BT474 with
Corning cDNA arrays. Hybridization
was carried out with a mixture of normal
human reference DNA (labeled with
green dye) and BT474 DNA (labeled with
red dye). Only a portion of the array
is shown.