258
Index
Lipohilic marine biotoxins, internal standards
(cont.)
lack of, 187
OA concentration, 190
PBI, 188
quadrupole analysis, 189
response variation, 193–195
retention time, 189
YTX measurements, 191
Lowest Observed Adverse Effect Level
(LOAEL), 78, 80
M
Manila clams (Venerupis philippinarum),
in MNV-1 bioaccumulation. See
Bioaccumulation, MNV-1
Marine biotoxins, bivalve molluscs
ARfD, 78–81
CCFFP, 76, 79–81
CCFH, 81
DA, 79, 80
LOAEL, 78, 80
OA, 80
risk assessments, 76–78
STX, 81
MBA. See Mouse bioassay (MBA)
Microalgae, storage and detoxification, 27
Ministry of Health, Labour andWelfare
(MHLW), 129, 130, 132
Mouse bioassay (MBA), 49, 50
Multiple samples strategy, 98–99
Murine norovirus (MNV-1)
amplification conditions, 168
cell culture and stocks, 167
clams contamination with, 169, 171
depuration facilities, 166
molluscs, 166–167
mussels contamination with, 169–170
primers and probes detection, 168
qRT-PCR controls, 168
quantification of, 169
RNA extraction, 168
sanitary controls, 165
Mytilus galloprovincialis (Mussels)
histopathology, mantle
cluster aggregation, 244, 245
follicles loss, 241, 242
gametogenic determination, 240
gonadal lesions, 241
haemocytes and phagocyte, 244, 246
karyorrhexis and karyolisis, 241, 242
multinucleated and hyperchromatic
cells, 241, 242
PAHs variation, 241, 243
PCA analysis, 244, 245
prevalence, 241, 243
sample collection and method, 240
statistical analysis, 240
mantle tissue, male
chemical analysis, 230
experimental design, 230–231
histopathological changes induce,
232–233
hydrocarbons, 229
PROBIT regression test, 231
tissue preparation, 231
in MNV-1 bioaccumulation (see
Bioaccumulation, MNV-1)
OA accumulation
CAT activity, 222, 223
CPR activity, 220, 222
Dinophysis acuminata, 217, 218
Dinophysis acuta, 217, 218
extractions, 219–220
GLO I activity, 221–223
GPXtot activity, 222, 223
GST activity, 221, 222
homogenization, 219
microalgae, 217
sample collection, 218
tar mixture exposure
chemical analysis, 235
mortality and histopathological effects,
235
PAH species, 232
test, 235
toxic accumulation, 232, 234
Mytulis edulis, DSP toxins, 206
N
National Direction of Aquatic Resources
(DINARA), 43, 44
National Fisheries Research and Development
Institute (NFRDI), 47–48
National monitoring program, 42
National Shellfish Sanitation Program (NSSP),
43
Neoplastic disorders, in mantle of Mytilus
galloprovincialis. See Mytilus
galloprovincialis (Mussels)
New Zealand aquaculture industry
case study, 65
council and Fonterra research
environmental monitoring, 69
faecal discharge consents, 69, 70
microbial source tracking, 70
Index
Lipohilic marine biotoxins, internal standards
(cont.)
lack of, 187
OA concentration, 190
PBI, 188
quadrupole analysis, 189
response variation, 193–195
retention time, 189
YTX measurements, 191
Lowest Observed Adverse Effect Level
(LOAEL), 78, 80
M
Manila clams (Venerupis philippinarum),
in MNV-1 bioaccumulation. See
Bioaccumulation, MNV-1
Marine biotoxins, bivalve molluscs
ARfD, 78–81
CCFFP, 76, 79–81
CCFH, 81
DA, 79, 80
LOAEL, 78, 80
OA, 80
risk assessments, 76–78
STX, 81
MBA. See Mouse bioassay (MBA)
Microalgae, storage and detoxification, 27
Ministry of Health, Labour andWelfare
(MHLW), 129, 130, 132
Mouse bioassay (MBA), 49, 50
Multiple samples strategy, 98–99
Murine norovirus (MNV-1)
amplification conditions, 168
cell culture and stocks, 167
clams contamination with, 169, 171
depuration facilities, 166
molluscs, 166–167
mussels contamination with, 169–170
primers and probes detection, 168
qRT-PCR controls, 168
quantification of, 169
RNA extraction, 168
sanitary controls, 165
Mytilus galloprovincialis (Mussels)
histopathology, mantle
cluster aggregation, 244, 245
follicles loss, 241, 242
gametogenic determination, 240
gonadal lesions, 241
haemocytes and phagocyte, 244, 246
karyorrhexis and karyolisis, 241, 242
multinucleated and hyperchromatic
cells, 241, 242
PAHs variation, 241, 243
PCA analysis, 244, 245
prevalence, 241, 243
sample collection and method, 240
statistical analysis, 240
mantle tissue, male
chemical analysis, 230
experimental design, 230–231
histopathological changes induce,
232–233
hydrocarbons, 229
PROBIT regression test, 231
tissue preparation, 231
in MNV-1 bioaccumulation (see
Bioaccumulation, MNV-1)
OA accumulation
CAT activity, 222, 223
CPR activity, 220, 222
Dinophysis acuminata, 217, 218
Dinophysis acuta, 217, 218
extractions, 219–220
GLO I activity, 221–223
GPXtot activity, 222, 223
GST activity, 221, 222
homogenization, 219
microalgae, 217
sample collection, 218
tar mixture exposure
chemical analysis, 235
mortality and histopathological effects,
235
PAH species, 232
test, 235
toxic accumulation, 232, 234
Mytulis edulis, DSP toxins, 206
N
National Direction of Aquatic Resources
(DINARA), 43, 44
National Fisheries Research and Development
Institute (NFRDI), 47–48
National monitoring program, 42
National Shellfish Sanitation Program (NSSP),
43
Neoplastic disorders, in mantle of Mytilus
galloprovincialis. See Mytilus
galloprovincialis (Mussels)
New Zealand aquaculture industry
case study, 65
council and Fonterra research
environmental monitoring, 69
faecal discharge consents, 69, 70
microbial source tracking, 70