196
T.S. Guldberg et al.
The conclusions one can draw from the study are somewhat limited owing to
the fact that the data set is small. However, the study undoubtedly represents a
starting point to improve the performance of MS detection as a valuable tool for
identification and quantification of marine biotoxins.
Calculations with the internal standards examined in this study are promising for
OA and Aza-1. DHO seems to be more suitable as an internal standard for OA than
for DTX-1, although DTX-1 elutes closer to DHO than OA.
The internal standard candidates examined in this initial work did not show
improvements related to response drift or matrix effects for DTX-1, PTX-2 or
YTX. The molecular structure and the chemical properties of YTX differ from the
other marine toxins, and thus make it more challenging to find one suitable internal
standard that matches all analytes in the methods. Further studies are required before
any definite conclusions can be drawn.
References
EU-Harmonised Standard Operating Procedure for determination of Lipophilic marine biotoxins
in molluscs by LC-MS/MS, version 4. Retrieved July 2011, from http://www.aesan.msps.es/en/
CRLMB/web/procedimientos crlmb/crlmb standard operating procedures.shtml
Gerssen A, Mulder PPJ, McElhinney MA, de Boer J (2009a) Liquid chromatography-tandem mass
spectrometry method for the detection of marine lipophilic toxins under alkaline conditions.
J Chromatogr A 1216:1421–1430
Gerssen A, McElhinney MA, Mulder PPJ, Bire R, Hess P, de Boer J (2009b) Solid phase extraction
for removal of matrix effects in lipophilic marine toxin analysis by liquid chromatographytandem mass spectrometry. Anal Bioanal Chem 394:1213–1226
Ito S, Tsukada K (2001) Matrix effect and correction by standard addition in quantitative
liquid chromatographic-mass spectrometric analysis of diarrhetic shellfish poisoning toxins.
J Chromatogr A 943:39–46
Regulation (EC) No 853/2004. Official Journal European Parliament and of the Council of 29
April 2004, p 99. Retrieved February 2011, from http://www.aesan.msps.es/en/CRLMB/web/
legislacion comunitaria/legislacion.shtml
Stobo LA, Lacaze JPCL, Scott AC, Gallacher S, Smith EA, Quilliam MA (2005) Liquid
chromatography with mass spectrometry – detection of lipophilic shellfish toxins. J AOAC
Int 88:1371–1382
These A, Scholz J, Preiss-Weigert A (2009) Sensitive method for the determination of lipophilic
marine biotoxins in extracts of mussels and processed shellfish by high-performance liquid
chromatography-tandem mass spectrometry based on enrichment by solid-phase extraction.
J Chromatogr A 1216:4529–4538
T.S. Guldberg et al.
The conclusions one can draw from the study are somewhat limited owing to
the fact that the data set is small. However, the study undoubtedly represents a
starting point to improve the performance of MS detection as a valuable tool for
identification and quantification of marine biotoxins.
Calculations with the internal standards examined in this study are promising for
OA and Aza-1. DHO seems to be more suitable as an internal standard for OA than
for DTX-1, although DTX-1 elutes closer to DHO than OA.
The internal standard candidates examined in this initial work did not show
improvements related to response drift or matrix effects for DTX-1, PTX-2 or
YTX. The molecular structure and the chemical properties of YTX differ from the
other marine toxins, and thus make it more challenging to find one suitable internal
standard that matches all analytes in the methods. Further studies are required before
any definite conclusions can be drawn.
References
EU-Harmonised Standard Operating Procedure for determination of Lipophilic marine biotoxins
in molluscs by LC-MS/MS, version 4. Retrieved July 2011, from http://www.aesan.msps.es/en/
CRLMB/web/procedimientos crlmb/crlmb standard operating procedures.shtml
Gerssen A, Mulder PPJ, McElhinney MA, de Boer J (2009a) Liquid chromatography-tandem mass
spectrometry method for the detection of marine lipophilic toxins under alkaline conditions.
J Chromatogr A 1216:1421–1430
Gerssen A, McElhinney MA, Mulder PPJ, Bire R, Hess P, de Boer J (2009b) Solid phase extraction
for removal of matrix effects in lipophilic marine toxin analysis by liquid chromatographytandem mass spectrometry. Anal Bioanal Chem 394:1213–1226
Ito S, Tsukada K (2001) Matrix effect and correction by standard addition in quantitative
liquid chromatographic-mass spectrometric analysis of diarrhetic shellfish poisoning toxins.
J Chromatogr A 943:39–46
Regulation (EC) No 853/2004. Official Journal European Parliament and of the Council of 29
April 2004, p 99. Retrieved February 2011, from http://www.aesan.msps.es/en/CRLMB/web/
legislacion comunitaria/legislacion.shtml
Stobo LA, Lacaze JPCL, Scott AC, Gallacher S, Smith EA, Quilliam MA (2005) Liquid
chromatography with mass spectrometry – detection of lipophilic shellfish toxins. J AOAC
Int 88:1371–1382
These A, Scholz J, Preiss-Weigert A (2009) Sensitive method for the determination of lipophilic
marine biotoxins in extracts of mussels and processed shellfish by high-performance liquid
chromatography-tandem mass spectrometry based on enrichment by solid-phase extraction.
J Chromatogr A 1216:4529–4538