15 Detection of Enteric Viruses in Shellfish
181
Fig. 15.2 Schematic diagram of steps in the GPTT procedure for virus extraction from shellfish
adequate controls are employed. In addition to the extraction controls mentioned
above, controls are needed for RT-PCR and include an internal amplification control
(e.g., plasmids, armored RNA, etc.); a positive RT-PCR control consisting of the
virus being sought (norovirus, hepatitis A virus, etc.), and one or more negative
RT-PCR controls. Together, the controls constitute an important part of the protocol.
It should be recognized that RT-PCR has several important limitations which cannot
be controlled for. Perhaps the greatest limitation is that RT-PCR detects total
181
Fig. 15.2 Schematic diagram of steps in the GPTT procedure for virus extraction from shellfish
adequate controls are employed. In addition to the extraction controls mentioned
above, controls are needed for RT-PCR and include an internal amplification control
(e.g., plasmids, armored RNA, etc.); a positive RT-PCR control consisting of the
virus being sought (norovirus, hepatitis A virus, etc.), and one or more negative
RT-PCR controls. Together, the controls constitute an important part of the protocol.
It should be recognized that RT-PCR has several important limitations which cannot
be controlled for. Perhaps the greatest limitation is that RT-PCR detects total