Composition of Salt Solutions and Culture Media
403
Locke's salt solution (Locke, 1895)
The concentrations are given as gll,OOO ml solution.
NaCI 9.0, KCI 0.42, CaCl 2 0.24, and NaHC0 3 0.2, glucose 1.0 made up to 1,000 ml with
distilled water.
Modified Alsever's anti-coagulant solution (Lebel et al., 1996)
115 mM glucose, 27 mM sodium citrate, 11.5 mM EDTA, and 3S2 mM NaCI.
Molluscan balanced salt solution (MMBSS; Machii and Wada, 1989)
The concentrations are given as gll,OOO ml solution.
NaCI26.22, KCI1.0S, MgS04 3.1S, MgCl2 2.20, CaCl2 1.12, NaHC03 0.30, NaH2P0 4 0.044,
and glucose 0.30, made up to 1,000 ml with distilled water.
Necco's physiological solution (Burnett et al., 1968)
The concentrations are given as gll,OOO ml solution.
NaCI 25.S00, KCI 0.644, CaCl2 0.644, MgCI2·6H20 3.0S0, KH 2 P04 0.020, Na 2 HP0 4 O.OSO,
NaHC03 0.200, Na 2 S04 0.920, sodium pyruvate 1.500, sodium fumarate 1.050, Lglutamine 1.090, and phenol red 0.020. The pH of the solution is adjusted to 7.5. The
antibiotics used in the solution are kanamycin 0.300 gIl, streptomycin 0.100 gIl, and
penicillin 0.100 gIl.
Nereis balanced salt solution (NBSS; Heacox et al., 1983)
To prepare NBSS, add 6.39 g NaCl, 1.00 g NaHC0 3 , 0.34 g KCI, and 0.169 g CaCI 2 ·2H 2 0
to 742.2 ml natural seawater. Dilute the solution with distilled water to attain the proper
osmolarity (920 mOsmollkg). Adjust the pH to between 7.5 and 7.7.
Octopus physiological solution (Necco and Martin, 1963)
The concentrations are given as g/l,OOO ml solution.
NaCl25.S, KCI 0.644, CaCl 2 0.644, MgCI 2 ·6H 2 0 3.0S, KH 2 P0 4 0.02, Na 2 HP0 4 O.OS, NaHC0 3
0.2, Na 2 S0 4 0.92, sodium pyruvate 1.5, sodium fumarate 1.05, L-glutamine 1.09, and
phenol red 0.01, made up to 1,000 ml with distilled water (pH 7.5).
Planarian salt solution (Murray, 1928)
The concentrations are given as gll,OOO ml solution.
NaCI3.0, CaCI 2 ·6H20 4.0, KCI 0.1, KN0 3 0.1, MgCl 2 1.0 g, MgS04 0.2, NaHC0 3 0.1, and
CaHP0 4 ·2Hp 0.1, made up to 1,000 ml with distilled water.
Rinaldini's salt solution (Rinaldini, 1959)
The concentrations are given as gll,OOO ml solution.
NaCIS.O, KCI 0.20, NaH2P04 0.044, NaHC0 3 1.0, glucose 1.0, and sodium citrate anhydrous O.SS, made up to 1,000 ml with distilled water.
Ringer's salt solution (Ringer, 1886)
The concentrations are given as gil ,000 ml solution.
NaCI 7.5, CaCl 2 0.125, KCI 0.1, and NaHC0 3 1.0, made up to 1,000 ml with distilled
water.
403
Locke's salt solution (Locke, 1895)
The concentrations are given as gll,OOO ml solution.
NaCI 9.0, KCI 0.42, CaCl 2 0.24, and NaHC0 3 0.2, glucose 1.0 made up to 1,000 ml with
distilled water.
Modified Alsever's anti-coagulant solution (Lebel et al., 1996)
115 mM glucose, 27 mM sodium citrate, 11.5 mM EDTA, and 3S2 mM NaCI.
Molluscan balanced salt solution (MMBSS; Machii and Wada, 1989)
The concentrations are given as gll,OOO ml solution.
NaCI26.22, KCI1.0S, MgS04 3.1S, MgCl2 2.20, CaCl2 1.12, NaHC03 0.30, NaH2P0 4 0.044,
and glucose 0.30, made up to 1,000 ml with distilled water.
Necco's physiological solution (Burnett et al., 1968)
The concentrations are given as gll,OOO ml solution.
NaCI 25.S00, KCI 0.644, CaCl2 0.644, MgCI2·6H20 3.0S0, KH 2 P04 0.020, Na 2 HP0 4 O.OSO,
NaHC03 0.200, Na 2 S04 0.920, sodium pyruvate 1.500, sodium fumarate 1.050, Lglutamine 1.090, and phenol red 0.020. The pH of the solution is adjusted to 7.5. The
antibiotics used in the solution are kanamycin 0.300 gIl, streptomycin 0.100 gIl, and
penicillin 0.100 gIl.
Nereis balanced salt solution (NBSS; Heacox et al., 1983)
To prepare NBSS, add 6.39 g NaCl, 1.00 g NaHC0 3 , 0.34 g KCI, and 0.169 g CaCI 2 ·2H 2 0
to 742.2 ml natural seawater. Dilute the solution with distilled water to attain the proper
osmolarity (920 mOsmollkg). Adjust the pH to between 7.5 and 7.7.
Octopus physiological solution (Necco and Martin, 1963)
The concentrations are given as g/l,OOO ml solution.
NaCl25.S, KCI 0.644, CaCl 2 0.644, MgCI 2 ·6H 2 0 3.0S, KH 2 P0 4 0.02, Na 2 HP0 4 O.OS, NaHC0 3
0.2, Na 2 S0 4 0.92, sodium pyruvate 1.5, sodium fumarate 1.05, L-glutamine 1.09, and
phenol red 0.01, made up to 1,000 ml with distilled water (pH 7.5).
Planarian salt solution (Murray, 1928)
The concentrations are given as gll,OOO ml solution.
NaCI3.0, CaCI 2 ·6H20 4.0, KCI 0.1, KN0 3 0.1, MgCl 2 1.0 g, MgS04 0.2, NaHC0 3 0.1, and
CaHP0 4 ·2Hp 0.1, made up to 1,000 ml with distilled water.
Rinaldini's salt solution (Rinaldini, 1959)
The concentrations are given as gll,OOO ml solution.
NaCIS.O, KCI 0.20, NaH2P04 0.044, NaHC0 3 1.0, glucose 1.0, and sodium citrate anhydrous O.SS, made up to 1,000 ml with distilled water.
Ringer's salt solution (Ringer, 1886)
The concentrations are given as gil ,000 ml solution.
NaCI 7.5, CaCl 2 0.125, KCI 0.1, and NaHC0 3 1.0, made up to 1,000 ml with distilled
water.