402
Appendix
Gey's salt solution (Gey, 1949)
The concentrations are given as gll,OOO ml solution.
NaCI 8.0, KCI 0.38, CaCl 2 0.13, MgClz-6H20 0.21, Na 2 HP0 4 ·12H 2 0 0.3, KH 2 P0 4 0.025,
NaHC0 3 1.0, and glucose 2.2, made up to 1,000 ml with distilled water.
Hydra culture solution (Loomis and Lenhoff, 1956)
Chemically defined solution: CaCl 2 1O- 3 M, NaHC0 3 1O- 3 M,
Convenient solution: NaHC0 3 20 g, disodium ethylendiaminetetraacetic acid 10 g/ tap
water 1,000 ml.
Insect Ringer's solutions (Yamasaki and Narahashi, 1963)
Cockroach
Silkworm
Japanese beetle
NaCI (g/l,OOO ml)
12.2
8.6
KCI (gl1,000 ml)
0.21
0.10
CaCI, (gil ,000 ml)
0.20
0.33
Buffer
*
**
*M/15 Na,HPO.: MIlS KH,PO. = 9: 1,50 ml, pH = 7.2
**M/15 Na,HPO. : MIlS KH,PO. = 4 : 6, 50 ml, pH = 6.6
Invertebrate Ringer's Solution
NaCI
KCI
CaCI,
(g/l,OOO ml) (gll,OOO ml) (gll,OOO ml)
Grasshopper
9.0
0.2
0.2
Silkworm
11.2
0.24
0.27
Drosophila
7.5
0.35
0.21
Earthworm
4.4
0.1
0.1
Seawater
25.0
2.6
2.3-3.0
crab
Freshwater
12.0
0.4
1.5
crab
NaHC03
(gll,OOO ml)
0.2
0.2
0.2
0.2
0.7
0.2
9.8
0.77
0.38
*
MgCI,
(gl1,000 ml)
1.6
0.2
Isotonic buffer for Echinococcus (IBE; Dieckmann and Frank, 1988)
NaCl121.00 mM, KCI 5.36 mM, MgS04 0.77 mM, MgCl 2 0.93 mM, Na 2 HP0 4 0.34 mM,
KH 2 P0 4 0.44 mM, glucose 5.56 mM, ethyleneglycol-bis-(~-aminoethylether) N,N'tetra acetic acid 0.20 mM, N-2-hydroxyethylpiperazine-N'-2-ethane-sulfonic acid
10.00 mM, pH 7.40.
Laverdure's physiological solution (Laverdure, 1971)
The concentrations are given as g/I,OOO ml solution.
CaCl 2 0.6, MgCI 2 ·6H 2 0 1.0, MgS04·7H 2 0 5.5, NaH2P0 4 1.0, KCI5.5, trehalose or glucose
1.6, made up to 1,000 ml with distilled water. The pH is adjusted to 7.0 with 5.5 %
NaHC03•
Leech Ringer's solution (Nicholls and Kuffler, 1965)
NaCl115 mM, KCl4 mM, CaCl 2 1.8 mM, Tris maleate buffered with NaOH 10 mM, and
glucose 11 mM.
Appendix
Gey's salt solution (Gey, 1949)
The concentrations are given as gll,OOO ml solution.
NaCI 8.0, KCI 0.38, CaCl 2 0.13, MgClz-6H20 0.21, Na 2 HP0 4 ·12H 2 0 0.3, KH 2 P0 4 0.025,
NaHC0 3 1.0, and glucose 2.2, made up to 1,000 ml with distilled water.
Hydra culture solution (Loomis and Lenhoff, 1956)
Chemically defined solution: CaCl 2 1O- 3 M, NaHC0 3 1O- 3 M,
Convenient solution: NaHC0 3 20 g, disodium ethylendiaminetetraacetic acid 10 g/ tap
water 1,000 ml.
Insect Ringer's solutions (Yamasaki and Narahashi, 1963)
Cockroach
Silkworm
Japanese beetle
NaCI (g/l,OOO ml)
12.2
8.6
KCI (gl1,000 ml)
0.21
0.10
CaCI, (gil ,000 ml)
0.20
0.33
Buffer
*
**
*M/15 Na,HPO.: MIlS KH,PO. = 9: 1,50 ml, pH = 7.2
**M/15 Na,HPO. : MIlS KH,PO. = 4 : 6, 50 ml, pH = 6.6
Invertebrate Ringer's Solution
NaCI
KCI
CaCI,
(g/l,OOO ml) (gll,OOO ml) (gll,OOO ml)
Grasshopper
9.0
0.2
0.2
Silkworm
11.2
0.24
0.27
Drosophila
7.5
0.35
0.21
Earthworm
4.4
0.1
0.1
Seawater
25.0
2.6
2.3-3.0
crab
Freshwater
12.0
0.4
1.5
crab
NaHC03
(gll,OOO ml)
0.2
0.2
0.2
0.2
0.7
0.2
9.8
0.77
0.38
*
MgCI,
(gl1,000 ml)
1.6
0.2
Isotonic buffer for Echinococcus (IBE; Dieckmann and Frank, 1988)
NaCl121.00 mM, KCI 5.36 mM, MgS04 0.77 mM, MgCl 2 0.93 mM, Na 2 HP0 4 0.34 mM,
KH 2 P0 4 0.44 mM, glucose 5.56 mM, ethyleneglycol-bis-(~-aminoethylether) N,N'tetra acetic acid 0.20 mM, N-2-hydroxyethylpiperazine-N'-2-ethane-sulfonic acid
10.00 mM, pH 7.40.
Laverdure's physiological solution (Laverdure, 1971)
The concentrations are given as g/I,OOO ml solution.
CaCl 2 0.6, MgCI 2 ·6H 2 0 1.0, MgS04·7H 2 0 5.5, NaH2P0 4 1.0, KCI5.5, trehalose or glucose
1.6, made up to 1,000 ml with distilled water. The pH is adjusted to 7.0 with 5.5 %
NaHC03•
Leech Ringer's solution (Nicholls and Kuffler, 1965)
NaCl115 mM, KCl4 mM, CaCl 2 1.8 mM, Tris maleate buffered with NaOH 10 mM, and
glucose 11 mM.