Appendix 1
Composition of Salt Solutions and Culture Media
(Listed in Alphabetical Order)
A Salt Solutions
Balanced salt solution (BSSO) for oyster (Tripp et al., 1966)
The concentrations are given as g/l,OOO ml solution.
NaCI23.50, KCI 0.67, CaCl z 1.10, MgCl z 2.03, MgS04 2.94, NaHC0 3 0.02, K z HP0 4 0.19,
glucose 0.50, trehalose 0.50, and phenol red 0.05, made up to 1,000 ml with distilled
water.
Buffered physiological solution with antibiotics (BPSA; Hansen, 1979)
Unless otherwise indicated, the concentrations are given as g/l,OOO ml solution.
NaCI 2.2, KCI 0.12, NazHP04 0.06, glucose 0.8, trehalose 0.8, penicillin 1,000,000 IV,
streptomycin 1.0, fungizone 0.0025, and chlortetracycline 0.05, made up to 1,000 ml
with distilled water.
Carlson's solution (Carlson et at, 1947)
The concentrations are given as g/l,OOO ml solution.
NaCI7.0, KCI 0.2, CaClz·HzO 0.2, MgClz·6HzO 0.1, NaHzP04 0.2, NaHC0 3 0.12, and glucose 8.0, made up to 1,000 ml with distilled water.
Chernin's balanced salt solution (BSS; Chernin, 1963)
Unless otherwise indicated, the concentrations are given as g/l,OOO ml solution.
NaCI 2.80, KCI 0.15, Na2HP04 0.07, MgS04·7H20 0.45, CaCI2·2H20 0.53, NaHC0 3 0.05,
glucose 1.00, trehalose 1.00, penicillin G 100,000 IV, streptomycin sulfate 0.1, and phenol red (0.4%) 5 ml, made up to 1,000 ml with distilled water.
Chiarandini's saline solution (Chiarandini, 1964)
The concentrations are given as g/l,OOO ml solution.
NaCI6.45, KCI 0.36, CaCI 2 ·2H z O 0.94, MgS0 4 ·7H z O 0.86, and NaHC0 3 1.54, made up to
1,000 ml with distilled water.
Crab saline (Cooke et at, 1989)
NaCl 440 mM, KCI 11.3 mM, CaCl z 13.3 mM, MgCl z 26 mM, Na Z S0 4 23 mM, HEPES
10 mM, pH adjusted to 7.4 with NaOH. A mixture of antibiotics (penicillin 100 IV/ml,
streptomycin 0.1 mg/ml, and fungizone 0.25 g/ml) is added to the Crab saline.
FPl Concentrated complex supplement (Domart-Coulon et al., 1994)
Insulin 500 Ilg/ml, hydrocortisone 10- 3 M, epidermal growth factor 250 ng/ml, platelet-derived growth factor 100 ng/ml, catalase 500 Ilg/ml, Ex-Cyte VLE (Pentex-Miles)
1 %, egg yolk extract 10%, prostaglandin F z 10- 7 M.
Composition of Salt Solutions and Culture Media
(Listed in Alphabetical Order)
A Salt Solutions
Balanced salt solution (BSSO) for oyster (Tripp et al., 1966)
The concentrations are given as g/l,OOO ml solution.
NaCI23.50, KCI 0.67, CaCl z 1.10, MgCl z 2.03, MgS04 2.94, NaHC0 3 0.02, K z HP0 4 0.19,
glucose 0.50, trehalose 0.50, and phenol red 0.05, made up to 1,000 ml with distilled
water.
Buffered physiological solution with antibiotics (BPSA; Hansen, 1979)
Unless otherwise indicated, the concentrations are given as g/l,OOO ml solution.
NaCI 2.2, KCI 0.12, NazHP04 0.06, glucose 0.8, trehalose 0.8, penicillin 1,000,000 IV,
streptomycin 1.0, fungizone 0.0025, and chlortetracycline 0.05, made up to 1,000 ml
with distilled water.
Carlson's solution (Carlson et at, 1947)
The concentrations are given as g/l,OOO ml solution.
NaCI7.0, KCI 0.2, CaClz·HzO 0.2, MgClz·6HzO 0.1, NaHzP04 0.2, NaHC0 3 0.12, and glucose 8.0, made up to 1,000 ml with distilled water.
Chernin's balanced salt solution (BSS; Chernin, 1963)
Unless otherwise indicated, the concentrations are given as g/l,OOO ml solution.
NaCI 2.80, KCI 0.15, Na2HP04 0.07, MgS04·7H20 0.45, CaCI2·2H20 0.53, NaHC0 3 0.05,
glucose 1.00, trehalose 1.00, penicillin G 100,000 IV, streptomycin sulfate 0.1, and phenol red (0.4%) 5 ml, made up to 1,000 ml with distilled water.
Chiarandini's saline solution (Chiarandini, 1964)
The concentrations are given as g/l,OOO ml solution.
NaCI6.45, KCI 0.36, CaCI 2 ·2H z O 0.94, MgS0 4 ·7H z O 0.86, and NaHC0 3 1.54, made up to
1,000 ml with distilled water.
Crab saline (Cooke et at, 1989)
NaCl 440 mM, KCI 11.3 mM, CaCl z 13.3 mM, MgCl z 26 mM, Na Z S0 4 23 mM, HEPES
10 mM, pH adjusted to 7.4 with NaOH. A mixture of antibiotics (penicillin 100 IV/ml,
streptomycin 0.1 mg/ml, and fungizone 0.25 g/ml) is added to the Crab saline.
FPl Concentrated complex supplement (Domart-Coulon et al., 1994)
Insulin 500 Ilg/ml, hydrocortisone 10- 3 M, epidermal growth factor 250 ng/ml, platelet-derived growth factor 100 ng/ml, catalase 500 Ilg/ml, Ex-Cyte VLE (Pentex-Miles)
1 %, egg yolk extract 10%, prostaglandin F z 10- 7 M.