404
Appendix
SA solution (Furuta and Shim ozawa, 1983)
Solution A: NaCI 2,486 mg, KCI 496 mg, CaCl z 6 mg, and MgClz 152 mg, made up to
500 ml with distilled water.
Solution B: fungizone 5 mg, kanamycin 1,000 mg, and streptomycin 1,000 mg, made
up to 40 ml with distilled water.
Ready-to-use solution: Solution A 50 ml, solution B 2 ml, 0.1 N NaOH 2 ml, phenol red
0.2 mg, and distilled water 44 ml.
Schistosoma salt solution (Weller and Wheeldon, 1982)
The concentrations are given as gll,OOO ml solution.
CaCl z ·2H z O 0040, KCI1.0, MgS04·7HzO 0040, NaC13.0, NaHC0 3 2.5, and NaH z P04 0.01,
made up to 1,000 ml with distilled water.
Snail physiological saline solution (Ivic et aI., 1995)
NaCI 100 mM, KCI 4 mM, MgCl z 3 mM, CaCl z 7 mM.
Tyrode's salt solution (Tyrode, 1910)
The concentrations are given as gll,OOO ml solution.
NaC18.0, KCI 0.2, CaCl z 0.2, NaHC0 3 1.0, NaH z P0 4 ·H z O 0.05, MgCl z ·6H z O 0.1, glucose
1.0 made up to 1,000 ml with distilled water.
van Harreveld's saline (van Harreveld, 1936)
The concentrations are given as gll,OOO ml solution.
NaCl 12, CaCl z 1.5, KCI 004, MgCl z 0.25, and NaHC03 0.2, made up to 1,000 ml with
distilled water.
WS washing solution (Rinkevich and Rabinovitz, 1993)
MgS0 4 ·7H z O 12.06 g (49 mM), CaClz 1.25 g (11.3 mM), NaCl 28.64 g (490 mM), solution of 20 mM HEPES (sodium salt N-2-hydroxyethylpiperazine-N' -2-ethenesulphonic
acid) 20 ml, and distilled water to 1,000 ml. The pH of the solution is adjusted to 7.3
with 1 N NaOH. The osmolarity is 1,200 mOsmol/kg. The solution should be sterilized
by filtration. The sterilized solution can be stored at 4°C until use.
B Culture Media
Barnacle organ culture medium IV (Fyhn and Costlow, 1975)
NaCl466 mM, KCl8 mM, CaCl z 20 mM, MgClz 12 mM, NaHC0 3 10 mM, glucose 0.1 %,
bovine embryo extract (Difco Laboratories) 1 %, and TC-Yeastolate (Difco Laboratories) 0.1%.
Basch and DiConza's medium C (Basch and DiConza, 1973)
The concentrations are given as mgll,OOO ml medium.
NaCl1,300, KCI 97, MgCl z ·6H z O 325, NazHP0 4 17, CaCl z ·2H z O 558, NaHC0 3 1,769, Llysine 5.8, L-histidine 2.0, L-arginine 204, L-aspartic acid 5.6, L-threonine 6.2, L-serine
12.0, L-glutamic acid 904, L-proline 5.8, glycine 4.8, L-alanine 4.8, L-valine 3.2, L-me-
Appendix
SA solution (Furuta and Shim ozawa, 1983)
Solution A: NaCI 2,486 mg, KCI 496 mg, CaCl z 6 mg, and MgClz 152 mg, made up to
500 ml with distilled water.
Solution B: fungizone 5 mg, kanamycin 1,000 mg, and streptomycin 1,000 mg, made
up to 40 ml with distilled water.
Ready-to-use solution: Solution A 50 ml, solution B 2 ml, 0.1 N NaOH 2 ml, phenol red
0.2 mg, and distilled water 44 ml.
Schistosoma salt solution (Weller and Wheeldon, 1982)
The concentrations are given as gll,OOO ml solution.
CaCl z ·2H z O 0040, KCI1.0, MgS04·7HzO 0040, NaC13.0, NaHC0 3 2.5, and NaH z P04 0.01,
made up to 1,000 ml with distilled water.
Snail physiological saline solution (Ivic et aI., 1995)
NaCI 100 mM, KCI 4 mM, MgCl z 3 mM, CaCl z 7 mM.
Tyrode's salt solution (Tyrode, 1910)
The concentrations are given as gll,OOO ml solution.
NaC18.0, KCI 0.2, CaCl z 0.2, NaHC0 3 1.0, NaH z P0 4 ·H z O 0.05, MgCl z ·6H z O 0.1, glucose
1.0 made up to 1,000 ml with distilled water.
van Harreveld's saline (van Harreveld, 1936)
The concentrations are given as gll,OOO ml solution.
NaCl 12, CaCl z 1.5, KCI 004, MgCl z 0.25, and NaHC03 0.2, made up to 1,000 ml with
distilled water.
WS washing solution (Rinkevich and Rabinovitz, 1993)
MgS0 4 ·7H z O 12.06 g (49 mM), CaClz 1.25 g (11.3 mM), NaCl 28.64 g (490 mM), solution of 20 mM HEPES (sodium salt N-2-hydroxyethylpiperazine-N' -2-ethenesulphonic
acid) 20 ml, and distilled water to 1,000 ml. The pH of the solution is adjusted to 7.3
with 1 N NaOH. The osmolarity is 1,200 mOsmol/kg. The solution should be sterilized
by filtration. The sterilized solution can be stored at 4°C until use.
B Culture Media
Barnacle organ culture medium IV (Fyhn and Costlow, 1975)
NaCl466 mM, KCl8 mM, CaCl z 20 mM, MgClz 12 mM, NaHC0 3 10 mM, glucose 0.1 %,
bovine embryo extract (Difco Laboratories) 1 %, and TC-Yeastolate (Difco Laboratories) 0.1%.
Basch and DiConza's medium C (Basch and DiConza, 1973)
The concentrations are given as mgll,OOO ml medium.
NaCl1,300, KCI 97, MgCl z ·6H z O 325, NazHP0 4 17, CaCl z ·2H z O 558, NaHC0 3 1,769, Llysine 5.8, L-histidine 2.0, L-arginine 204, L-aspartic acid 5.6, L-threonine 6.2, L-serine
12.0, L-glutamic acid 904, L-proline 5.8, glycine 4.8, L-alanine 4.8, L-valine 3.2, L-me-