354
Related Techniques
Setting up
the
microscope
Focusing
Exposure
Film
development
Film
fixation
Washing
the film
1. Set up the microscope so that it is ready to take photographs.
2. Place a culture flask on the microscope stage.
3. Select a field in which the object to be photographed is included.
1. Adjust the focus. If a photographic apparatus is being used, check the
focus using the focusing lens on the apparatus. If an automatic camera is being used, focusing the microscope on the object to be photographed usually focuses the camera directly; if not, focus on the object to be photographed through the camera view finder.
1. Exposure may be determined automatically. If the photographic apparatus being used is an older model, the light exposure meter and
shutter system may be separated. If this is the case, set the shutter
speed according to the light exposure meter.
2. The use of a green filter is usually recommended for a phase-contrast
optical system. However, if the exposure time when using the green
filter increases to more than several seconds, remove the filter. Theoretically, monochromatic illumination produces sharp focus; however,
if the exposure time is prolonged, the "fuzziness" of an image, caused
by vibrations, will increase.
~-o~ Comments
If a highly magnified photograph is required, a high-magnification objective lens, not an eye piece, should be used.
It is recommended that the scales of an objective micrometer are also
photographed to enable determination of the real size of photographed
objects in the enlarged print.
Minicopy, or a similar high -contrast film, should be developed using ND4 developer, which is a mild contrast developer.
1. Place the film in a developing tank.
2. Pour the developer into the tank.
3. Slowly agitate the developer continuously for 10 min at 20°e.
4. Discard the developer, and pour tap water into the tank to wash the
film.
1. Discard the water, and pour the film fixative into the tank.
2. Leave the film in the fixative for approximately 10 min, stirring the
fixative occasionally.
1. Discard the fixative, and wash the film in the tank with running water.
Hereafter, the lid of the developing tank can be opened. Wash the film
with running water for 15 min.
2. Wash the film in distilled water or dip it into Driwel solution.
3. Remove the film from the tank, and hang it up to let it air dry.
Related Techniques
Setting up
the
microscope
Focusing
Exposure
Film
development
Film
fixation
Washing
the film
1. Set up the microscope so that it is ready to take photographs.
2. Place a culture flask on the microscope stage.
3. Select a field in which the object to be photographed is included.
1. Adjust the focus. If a photographic apparatus is being used, check the
focus using the focusing lens on the apparatus. If an automatic camera is being used, focusing the microscope on the object to be photographed usually focuses the camera directly; if not, focus on the object to be photographed through the camera view finder.
1. Exposure may be determined automatically. If the photographic apparatus being used is an older model, the light exposure meter and
shutter system may be separated. If this is the case, set the shutter
speed according to the light exposure meter.
2. The use of a green filter is usually recommended for a phase-contrast
optical system. However, if the exposure time when using the green
filter increases to more than several seconds, remove the filter. Theoretically, monochromatic illumination produces sharp focus; however,
if the exposure time is prolonged, the "fuzziness" of an image, caused
by vibrations, will increase.
~-o~ Comments
If a highly magnified photograph is required, a high-magnification objective lens, not an eye piece, should be used.
It is recommended that the scales of an objective micrometer are also
photographed to enable determination of the real size of photographed
objects in the enlarged print.
Minicopy, or a similar high -contrast film, should be developed using ND4 developer, which is a mild contrast developer.
1. Place the film in a developing tank.
2. Pour the developer into the tank.
3. Slowly agitate the developer continuously for 10 min at 20°e.
4. Discard the developer, and pour tap water into the tank to wash the
film.
1. Discard the water, and pour the film fixative into the tank.
2. Leave the film in the fixative for approximately 10 min, stirring the
fixative occasionally.
1. Discard the fixative, and wash the film in the tank with running water.
Hereafter, the lid of the developing tank can be opened. Wash the film
with running water for 15 min.
2. Wash the film in distilled water or dip it into Driwel solution.
3. Remove the film from the tank, and hang it up to let it air dry.