37 Microscope Photography
355
Images photographed using Minicopy film and developed with ND-4 Printing
developer are high contrast images. It is recommended that no. 2, sometimes no. 3, photographic paper is used for printing these images.
1. Using an enlarger, project the image from the negative film onto the
area where the photographic paper will be placed.
2. Select the area to be printed.
3. Enlarge the image to be printed as much as possible by cropping any
unnecessary surroundings.
4. Expose the image onto the photographic paper.
5. Develop the photograph.
6. Stop the development by transferring the paper into 1.5% acetic acid
for 3 min.
7. Fix the paper using a fixative.
1. Wash the paper in running water for more than 15 min.
Finishing
2. Immerse the paper in Driwel solution.
3. Air dry the paper.
Results
Photographs with various tones can be obtained by careful selection of
photographic paper and by adjusting the exposure time. If the paper is
kept too long in the developer, a yellow stain will develop on the photograph.
Comments
When the real distance between two parts on an enlarged print needs to
be determined, compare the negative film of cells and scales from an
objective micrometer. Thus, the real distance can be estimated.
Color photographs can be taken through the microscope. This is particularly important for recording stained tissues or cell preparations. The
method used for color photography is the same as that for taking black
and white photographs, but the film is usually sent to a professional film
developer.
Recently, digital cameras have become popular. Microscope photography can be undertaken with a digital camera in a manner similar to
that using an ordinary camera. However, because digital cameras do not
use film, it is not necessary to develop the film. In addition, images taken
with a digital camera can be transfered to a computer and can be modified by a computer prior to printing. The system for digital recording of
the microscopic image is developing quickly in a recent year. For the
model of such system, consult with microscope manufacturer.
355
Images photographed using Minicopy film and developed with ND-4 Printing
developer are high contrast images. It is recommended that no. 2, sometimes no. 3, photographic paper is used for printing these images.
1. Using an enlarger, project the image from the negative film onto the
area where the photographic paper will be placed.
2. Select the area to be printed.
3. Enlarge the image to be printed as much as possible by cropping any
unnecessary surroundings.
4. Expose the image onto the photographic paper.
5. Develop the photograph.
6. Stop the development by transferring the paper into 1.5% acetic acid
for 3 min.
7. Fix the paper using a fixative.
1. Wash the paper in running water for more than 15 min.
Finishing
2. Immerse the paper in Driwel solution.
3. Air dry the paper.
Results
Photographs with various tones can be obtained by careful selection of
photographic paper and by adjusting the exposure time. If the paper is
kept too long in the developer, a yellow stain will develop on the photograph.
Comments
When the real distance between two parts on an enlarged print needs to
be determined, compare the negative film of cells and scales from an
objective micrometer. Thus, the real distance can be estimated.
Color photographs can be taken through the microscope. This is particularly important for recording stained tissues or cell preparations. The
method used for color photography is the same as that for taking black
and white photographs, but the film is usually sent to a professional film
developer.
Recently, digital cameras have become popular. Microscope photography can be undertaken with a digital camera in a manner similar to
that using an ordinary camera. However, because digital cameras do not
use film, it is not necessary to develop the film. In addition, images taken
with a digital camera can be transfered to a computer and can be modified by a computer prior to printing. The system for digital recording of
the microscopic image is developing quickly in a recent year. For the
model of such system, consult with microscope manufacturer.