Chapter 23
Prochordata
Subprotocol 1
Gonads of Protochordates (Tunicata)
Materials
- Dissecting tray
- Scissors
- Forceps
- Pasteur pipettes
- Culture vessels
- Basic culture medium (seawater)
- Molgula manhattensis
Procedure
1. Aseptically excise gonads from the animal.
2. Place the excised organs in the culture medium.
Results
According to Sengel and Kieny (1962), the gonads cultured in this way
survived for 7 days.
Co culture with neural gland-nerve ganglion-vibratile organ improved the culture. Under these conditions, testes were not altered, but
oogenesis proceeded to some extent.
References
PROTOCOL
Equipment
Solutions
Animals
Sengel, P. and Kieny, M. (1962) Action de divers liquides nutritifs et du complexe «glande nueraleganglion nerveux-organe vibratile» sur les gonades de Molguia manhattensis (Tunicier
Ascidiace) cultivees in vitro. C. R. Acad. Sci. 254,1682-1684.