10
General Methods
Flow
cytometry
Magnetic
stirrer
A
Fig. 13. A filter system. A, positive and negative pressure pump; B, a trap that has an outlet with a stopcock; C, the membrane filter
unit
Fig. 14. A small pressure and vacuum pump. +, pressure (air outlet); -, vacuum (air inlet)
in suspension. With this apparatus, it is possible to count cells differentially according to cell volume. Rather than using a cell-counting apparatus, a hemocytometer may be used. There are several types ofhemocytometer available, such as Thoma, Burker-Turk, and Neubauer (for example Ikemoto Rika).
The flow cytometer (Fig. 18) labels cells with fluorescent material and
analyzes differences between cells.
A magnetic stirrer is convenient for the preparation of physiological salt
solutions or culture media. When sterilizing Teflon-coated magnetic bars,
an autoclave should be used rather than a dry oven, because the latter
may reduce the magnetic force of the bar. Magnetic stirrers are used for
spinner culture of suspended cells. For this purpose, a rotating field-type
driver or a belt driver from an external motor should be used to preclude heating from the stirrer motor. The stirrer should have a meter so
that rotation speed can be read.
3 Tools
Pipette Short pipettes (approximately 23.0 em long), graduated to the tip with
the maximum point of the scale at the top, are preferable for working in
General Methods
Flow
cytometry
Magnetic
stirrer
A
Fig. 13. A filter system. A, positive and negative pressure pump; B, a trap that has an outlet with a stopcock; C, the membrane filter
unit
Fig. 14. A small pressure and vacuum pump. +, pressure (air outlet); -, vacuum (air inlet)
in suspension. With this apparatus, it is possible to count cells differentially according to cell volume. Rather than using a cell-counting apparatus, a hemocytometer may be used. There are several types ofhemocytometer available, such as Thoma, Burker-Turk, and Neubauer (for example Ikemoto Rika).
The flow cytometer (Fig. 18) labels cells with fluorescent material and
analyzes differences between cells.
A magnetic stirrer is convenient for the preparation of physiological salt
solutions or culture media. When sterilizing Teflon-coated magnetic bars,
an autoclave should be used rather than a dry oven, because the latter
may reduce the magnetic force of the bar. Magnetic stirrers are used for
spinner culture of suspended cells. For this purpose, a rotating field-type
driver or a belt driver from an external motor should be used to preclude heating from the stirrer motor. The stirrer should have a meter so
that rotation speed can be read.
3 Tools
Pipette Short pipettes (approximately 23.0 em long), graduated to the tip with
the maximum point of the scale at the top, are preferable for working in