12 Arthropods Other than Insects
135
justed to 760±10 mOsmollkg with a mixture of salts. The cultures were
incubated at 28°C. Cell growth was observed after 12 h, and a sheet of
confluent cells was formed within 24-48 h.
Subprotocol 8
Crustacean (Crustacea) Testicular Cells
• • Materials
- Centrifuge and centrifuge tubes
- Forceps
- Scissors
- Curved iris scissors
- Petri dishes
- Plastic tissue flasks (Corning)
Equipment
- Culture medium (medium 199 with the addition of 2.8 gIl NaCI, Solutions
190 mgll MgCI2, 40 mgll proline for crayfish, and the addition of 22.0 gil
NaCI, 200 mgll MgCl 2 and 60 mgll proline for lobster; pH 7.5)
- Wesco dyne (iodophor), (West Agro)
Disinfectant
- Lobster Homarus americanas
Animals
- Crayfish Pacifasticus leniusculus
Fig. 10. Monolayer of cells migrated from the Oka gland of the shrimp Penaeus
stylirostris.x 100. From Nadala,E.C., et aI., "Primary culture oflymphoid, nerve, and
ovary cells from Penaeus stylrostris and Penaeus vannamei", In Vitro Cellular and
Developmental Biology-Animal, Vo1.29 A, pp621. Copyright © 1993 by the Society
for In Vitro Biology (formerly the Tissue Culture Association), Reproduced with
permission of the copyright owner