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Methods for Setting Up Primary Cultures Specific to Animal Groups
Cardiac stomach
Fig. 11. Internal anatomy of a lobster in lateral view
• • Procedure
Vas deferens
Fig. 12. A 1-month-old primary
cell culture of crayfish testes. x 110.
From Brody, M.D. and Chang, E.S.,
1989, with permission
Sterilization 1. Surface sterilize the animals with 20% Wesco dyne for 10 min.
2. Rinse the animals with 70% ethanol.
3. Air-dry the animals in a laminar flow cabinet for 5 min.
Dissection 1. Split the dorsal seam toward the rostrum and pull each half of the
carapace free of the thorax.
2. Remove testicular tissues carefully using forceps, avoiding contact with
the gut and midgut gland and other sources of contamination (Fig. 11).
3. Transfer testicular tissues into a drop of medium in a sterile petri dish.
Methods for Setting Up Primary Cultures Specific to Animal Groups
Cardiac stomach
Fig. 11. Internal anatomy of a lobster in lateral view
• • Procedure
Vas deferens
Fig. 12. A 1-month-old primary
cell culture of crayfish testes. x 110.
From Brody, M.D. and Chang, E.S.,
1989, with permission
Sterilization 1. Surface sterilize the animals with 20% Wesco dyne for 10 min.
2. Rinse the animals with 70% ethanol.
3. Air-dry the animals in a laminar flow cabinet for 5 min.
Dissection 1. Split the dorsal seam toward the rostrum and pull each half of the
carapace free of the thorax.
2. Remove testicular tissues carefully using forceps, avoiding contact with
the gut and midgut gland and other sources of contamination (Fig. 11).
3. Transfer testicular tissues into a drop of medium in a sterile petri dish.