Contents XIII
Chapter 32 Karyotype Analysis .......................................................... . 321
Chapter 33 Identification of Cell Lines .............................................. . 325
1 Isozyme Analysis by Agar Gel Electrophoresis ..................................... . 325
2 Phosphoglucoisomerase Isozyme Pattern on Isoelectric
Focusing ...................................................................................................... . 328
3 DNA Amplification Fingerprinting (DAF) ............................................. . 329
4 RAPD-PCR .................................................................................................. . 331
Chapter 34 Viability of Cells ............................................................... . 337
1 Vital Staining Method ............................................................................... . 337
2 Dye-Exclusion Method ............................................................................. . 337
Chapter 35 Viable Cell Enumeration ................................................. . 341
1 Hemocytometer Count ............................................................................. . 341
2 Counting Cells Using a Grid Projected onto a Microscope Field ........ . 342
3 MTT Method ............................................................ , ................................. . 343
4 Protein Measurement ................................................................................ . 344
Chapter 36 Growth Rate ..................................................................... . 347
1 Growth Curves ........................................................................................... . 347
2 Population Doubling Time ....................................................................... . 350
Chapter 37 Microscope Photography ................................................ . 353
Chapter 38 Virus Inoculation-Plaque Assay .................................... . 357
1 Preparation of Inoculum from Hemolymph .......................................... . 357
2 Preparation of Inoculum from Whole Bodies or Tissues ..................... . 358
3 Preparation of Inoculum from Isolated Occlusion Bodies ................... . 359
4 Preparation of Inoculum from an Infected Cell Culture ...................... . 360
5 Qualitative Inoculation ............................................................................. . 360
6 Quantitative Inoculation (Infectivity and Multiplicity of Infection) .. . 361
7 Plaque Assay Method ................................................................................ . 363
Chapter 39 Examination of Drug Effects ......................................... .. 367
Chapter 40 Cell Fusion ........................................................................ . 369
1 Cell Fusion by Viruses ............................................................................... . 369
2 Cell Fusion with Polyethyleneglycol ...................................................... .. 370
3 Electrofusion of Cells ............................................................................... .. 371
4 Method for the Selection of Hybrid Cells: Use of Auxotrophic
Cells ............................................................................................................. . 374
5 Method for the Selection of Hybrid Cells: Use of ts Mutants .............. . 375
Chapter 41 Gene Technology ............................................................. . 379
1 DNA-Calcium Phosphate Coprecipitation Method ............................. .. 379
2 Polybrene-DMSO Method ....................................................................... .. 380
3 Lipofection Method .................................................................................. .. 381
4 Electroporation Method .......................................................................... .. 382
5 Selection of Transfected Cells with G418 .............................................. .. 383