XIV
Contents
6 Selection of Transfected Cells with Puromycin ...................................... 384
7 Selection of Transfected Cells with Hygromycin B................................ 384
8 Selection of Transfected Cells with a-Amanitin .................................... 385
Chapter 42 Large-Scale Cell Culture .................................................. 387
1 Anchorage-Dependent Cell Culture ......................................................... 388
2 Shaking Flask Culture ................................................................................ 390
3 Spinner Culture ........................................................................................... 390
4 Stirred Tank Bioreactor Culture ................................................................ 391
5 Internal Membrane Perfusion Culture ..................................................... 393
6 External Membrane Perfusion Culture .................................................... 394
7 Air-Sparged Bioreactor Culture (Air-Lift Bioreactor Culture) .............. 395
8 Immobilization of Cells by the Microcapsule Method .......................... 396
Appendix
1 Composition of Salt Solutions and Culture Media ................................. 401
2 List of Reported Continuous Invertebrate Cell Lines ............................ 421
3 Commercially Available Culture Medium Products and
List of Suppliers .......................................................................................... 435
Subject Index ..................................................................................................... 439