Box 16.5 (continued)
(continued)
Box 16.5 (continued)
(continued)
OH
H
alkL (uptake)
a
alkN (chemotaxis?)
alkB
alkG
alkT
alkJ
alkK
alkH
alkF
β β‐oxidation
n‐alkanes +
alkS
+ regulation
alkST
alkBFGHJKL (alkN)
O
O
OH
O
S‐CoA
Outer
Membrane
Inner
Membrane
alkB
alkF
alkG
alkH
alkJ
alkK
alkL
alkN
tnpA1
alkT
alkS
Genes involved, or presumably involved,
in alkane degradation
Complete or partial transposase genes
Other potential coding regions
2 Kb
b
Box Fig. 16.10 Metabolic pathway of n-alkane degradation in
Pseudomonas putida GPo1. (a) Role and cellular localization
of Alk enzymes and regulation of the alk genes: AlkB (alkane
hydroxylase), AlkF and AlkG (rubredoxins), AlkH (aldehyde
dehydrogenase), AlkJ (alcohol dehydrogenase), AlkK (acyl-CoA
synthetase), AlkL (outer-membrane protein functioning as an
alkane uptake facilitator), AlkN (methyl-accepting transducer
protein, putative chemotactic transducer for alkanes), AlkT
(rubredoxin reductase), and AlkS (positive regulator of the expression of the alkBFGHJKL operon) (Modified from van Hamme et al.
2003). (b) Genomic map showing the alk genes organization
(Modified from van Beilen et al. 2001)
16 Applied Microbial Ecology and Bioremediation
725