661
1.5 % (w/v) agar
distilled water to 1 l
pH 8.6; heat to dissolve, do not autoclave
Waltman-Shotts Medium for the Isolation of Yersinia ruckeri
(Waltman and Shotts 1984 )
0.2 % (w/v) tryptone
0.2 % (w/v) yeast extract
1.0 % (v/v) Tween 80
0.5 % (w/v) sodium chloride
0.01 % (w/v) calcium chloride hydrated
0.0003 % (w/v) bromothymol blue
1.5 % (w/v) agar
pH 7.4; sterilise at 121 °C for 15 min, cool to 50 °C and add fi lter sterilised sucrose
to 0.5 % (w/v).
Yeast Extract Glucose Agar (Michel et al. 1997 )
2.5 % (w/v) nutrient broth No. 2[Oxoid]
0.3 % (w/v) yeast extract
0.5 % (w/v) glucose
1.5 % (w/v) agar
pH 6.8
Yersinia ruckeri Selective Medium (Furones et al . 1993 )
TSA
1 % (w/v) SDS
100 μg/ml of coomassie brilliant blue
100 μg/ml of Congo red
References
Álvarez B, Guijarro JA (2007) Recovery of Flavobacterium psychrophilum viable cells using a
charcoal- based solid medium. Lett Appl Microbiol 44:569–572
Anacker RL, Ordal EJ (1959) Studies on the myxobacterium Chondrococcus columnaris. 1.
Serological typing. J Bacteriol 78:25–32
References