660
1.0 % (w/v) sodium chloride
0.1 % (w/v) ferric citrate
0.004 % (w/v) bromothymol blue
0.004 % (w/v) thymolblue
1.4 % (w/v) agar
pH 8.6; after boiling to dissolve the ingredients the medium will not require further
sterilisation.
Todd-Hewitt Broth
1 % (w/v) infusion from 450 g of fat-free minced beef
2 % (w/v) tryptone
0.2 % (w/v) dextrose
0.2 % (w/v) sodium bicarbonate
0.2 % (w/v) sodium chloride
0.04 % (w/v) disodium phosphate, anhydrous
pH 7.8
Sterilise at 115 °C/10 min.
Vibrio alginolyticus Agar (VAL)
03.7 % (w/v) Brain heart infusion
8.5 % (w/v) sodium chloride
1.5 % (w/v) sucrose
0.1 % (w/v) oxgall
0.002 % (w/v) bromocresol purple
1.5 % (w/v) agar
distilled water to 1 l
Boil, cool to 55 °C, adjust to pH 7.4 ± 0.2; do not autoclave
Vibrio anguillarum Medium (VAM)
1.5(w/v) sorbitol
0.4 % (w/v) yeast extract
0.5 % (w/v) bile salts
3.5 % (w/v) sodium chloride
0.001 % (w/v) ampicillin
0.004 % (w/v) cresol red
0.004 % (w/v) bromothymol blue
12 Isolation/Detection