659
Skimmed Milk Agar
0.05 % (w/v) yeast extract
1.0 % (w/v) skimmed milk powder
1.0 % (w/v) agar No. 3
pH 7.2
sterilise at 115 °C/15 min.
TCY Medium (Hikida et al. 1979 )
0.1 % (w/v) casamino acids
0.1 % (w/v) tryptone
0.02 % (w/v) yeast extract
0.1 % (w/v) calcium chloride
1.08 % (w/v) magnesium chloride
0.07 % (w/v) potassium chloride
3.13 % (w/v) sodium chloride
pH 7.0–7.2
Thioglycollate Broth
0.05 % (w/v) L-cystine
0.25 % (w/v) sodium chloride
0.5 % (w/v) dextrose
0.5 % (w/v) yeast extract
1.5 % (w/v) pancreatic digest of casein
0.05 % 9 w/v) sodium thioglycollate
pH 7.1
Sterilise at 121 °C/15 min
Thiosulphate Citrate Bile Salt Sucrose Agar (TCBS)
0.5 % (w/v) yeast extract
1.0 % (w/v) peptone
1.0 % (w/v) sodium thiosulphate
1.0 % (w/v) sodium citrate
0.8 % (w/v) ox bile
2.0 % (w/v) sucrose
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens