653
Loeffl er (Serum) Medium
75 % (v/v) horse serum
0.25 % (w/v) Lab-lemco powder
0.25 % (w/v) peptone
0.25 % (w/v) dextrose
0.125 % (w/v) sodium chloride
distilled water to 1 l.
Löwenstein-Jensen Medium
0.15 % (w/v) potassium hydrogen phosphate
o.15 % (w/v) magnesium sulphate hydrated
0.037 % (w/v) magnesium citrate
0.22 % (w/v) asparagine
0.73 % (v/v) glycerol
1.83 % (w/v) potato starch
60.97 % (v/v) fresh egg mixture (whites and yolks)
0.024 % (w/v) malachite green
inspissated (~80 °C for 1 h)
MacConkey Agar
2 % (w/v) peptone
1 % (w/v) lactose
0.5 % (w/v) bile salts
0.5 % (w/v) sodium chloride
0.0075 % (w/v) neutral red
1.3 % (w/v) agar
pH 7.4
Sterilise at 121 °C/15 min.
Medium K (Mudarris and Austin 1988 )
0.5 % (w/v) beef extract
0.6 % (w/v) casein
0.2 % (w/v) tryptone
0.1 % (w/v) yeast extract
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens
Loeffl er (Serum) Medium
75 % (v/v) horse serum
0.25 % (w/v) Lab-lemco powder
0.25 % (w/v) peptone
0.25 % (w/v) dextrose
0.125 % (w/v) sodium chloride
distilled water to 1 l.
Löwenstein-Jensen Medium
0.15 % (w/v) potassium hydrogen phosphate
o.15 % (w/v) magnesium sulphate hydrated
0.037 % (w/v) magnesium citrate
0.22 % (w/v) asparagine
0.73 % (v/v) glycerol
1.83 % (w/v) potato starch
60.97 % (v/v) fresh egg mixture (whites and yolks)
0.024 % (w/v) malachite green
inspissated (~80 °C for 1 h)
MacConkey Agar
2 % (w/v) peptone
1 % (w/v) lactose
0.5 % (w/v) bile salts
0.5 % (w/v) sodium chloride
0.0075 % (w/v) neutral red
1.3 % (w/v) agar
pH 7.4
Sterilise at 121 °C/15 min.
Medium K (Mudarris and Austin 1988 )
0.5 % (w/v) beef extract
0.6 % (w/v) casein
0.2 % (w/v) tryptone
0.1 % (w/v) yeast extract
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens