652
0.02 % (w/v) sodium acetate
0.9 % (w/v) agar
pH 7.2–7.4
sterilise at 121 °C/15 min, allow to cool, and add fi lter sterilised antibiotic
solutions:
5 μg/ml of neomycin sulphate and 10 IU/ml of polymyxin B.
FLP Medium (for Fla. psychrophilum ; Cepeda et al. 2004 )
0.05 % (w/v) glucose
0.02 % (w/v) CaCl 2 .2H 2 O
0.05 % (w/v) MgSO 4 .7H 2 O
0.4 % (w/v) tryptone
0.04 % (w/v) yeast extract
1 % (w/v) agar
pH 7.2–7.4
sterilise at 121 °C/15 min
Kidney Disease Medium 2 (KDM2)
1.0 % (w/v) peptone
0.05 % (w/v) yeast extract
0.1 % (w/v) L-cysteine hydrochloride
1.5 % (w/v) agar
pH 6.5; sterilise at 121 °C for 15 min, cool to 45 °C, and add 20 % (v/v) sterile foetal
calf serum.
L-F Medium
– 1.0 % (w/v) brain heart infusion
– 10.0 % (w/v) sucrose
– 0.5 % (w/v) yeast extract
– 1.0 % (w/v) agar No. 3
– Sterilise at 115 °C/20 min
10 % (v/v) horse serum, inactivated by heating at 60 °C/30 min.
Cool the medium and warm the horse serum to ~50 °C, mix, and pour as plates.
12 Isolation/Detection
0.02 % (w/v) sodium acetate
0.9 % (w/v) agar
pH 7.2–7.4
sterilise at 121 °C/15 min, allow to cool, and add fi lter sterilised antibiotic
solutions:
5 μg/ml of neomycin sulphate and 10 IU/ml of polymyxin B.
FLP Medium (for Fla. psychrophilum ; Cepeda et al. 2004 )
0.05 % (w/v) glucose
0.02 % (w/v) CaCl 2 .2H 2 O
0.05 % (w/v) MgSO 4 .7H 2 O
0.4 % (w/v) tryptone
0.04 % (w/v) yeast extract
1 % (w/v) agar
pH 7.2–7.4
sterilise at 121 °C/15 min
Kidney Disease Medium 2 (KDM2)
1.0 % (w/v) peptone
0.05 % (w/v) yeast extract
0.1 % (w/v) L-cysteine hydrochloride
1.5 % (w/v) agar
pH 6.5; sterilise at 121 °C for 15 min, cool to 45 °C, and add 20 % (v/v) sterile foetal
calf serum.
L-F Medium
– 1.0 % (w/v) brain heart infusion
– 10.0 % (w/v) sucrose
– 0.5 % (w/v) yeast extract
– 1.0 % (w/v) agar No. 3
– Sterilise at 115 °C/20 min
10 % (v/v) horse serum, inactivated by heating at 60 °C/30 min.
Cool the medium and warm the horse serum to ~50 °C, mix, and pour as plates.
12 Isolation/Detection