651
crystal violet may also be incorporated to suppress contaminants.
inspissated (~80 °C for 1 h)
Edwardsiella tarda (ET) Medium (Lindquist 1991 ; Castro et al.
2011 )
4.0 % (w/v) MacConkey agar base
0.1 % (w/v) yeast extract
0.45 % (w/v) agar [in addition to that contained in the MacConkey agar]
900 ml of distilled water
Autoclave (121 °C/15 min) and cool. Then add fi lter-sterilised solutions [100 ml to
make 1.0 l in total] of:
A
0.2 % (w/v) glucose
0.5 % (w/v) sucrose
0.5 % (w/v) xylose
1.0 % (w/v) L-lysine
0.68 % (w/v) sodium thiosulphate
0.08 % (w/v) ferric ammonium sulphate
100 ml of distilled water
B
10 ml of colistin (1 mg/ml)
Emerson Agar
0.4 % (w/v) beef extract
0.1 % (w/v) yeast extract
0.4 % (w/v) peptone
1 % (w/v) dextrose
0.25 % (w/v) sodium chloride
2 % (w/v) agar
pH 7.0
Sterilise at 121 °C/15 min.
Flavobacterium columnare Selective Medium (Fijan 1969 )
0.02 % (w/v) beef extract
0.05 % (w/v) tryptone
0.05 % (w/v) yeast extract
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens