650
Improved Growth Medium for Fla. psychrophilum (Daskalov
et al. 1999 )
Cytophaga agar/broth supplemented with:
0.05 % (w/v) D(+) galactose
0.05 % (w/v) D(+) glucose
0.05 % (w/v) skimmed milk powder
The supplement were prepared as 10 % (w/v) solutions, and fi ltered separately
through 0.22 μm porosity fi lters, and added to molten, cooled medium
LG Agar for Lactococcus garvieae (Chang et al. 2014 )
5 % (w/v) sucrose
3 % (w/v) oxgall (Difco)
0.9 % (w/v) proteose peptone No. 3
0.6 % (w/v) pancreatic digest of casein
0.5 % (w/v) proteose peptone
0.1 % (w/v) dextrose
1.5 % (w/v) agar
The ingredients were dissolved, adjusted to pH 7.0 before sterilizing at
121 °C/15 min.
The medium was cooled to 55 °C, and 8 ml/l of 1 % (w/v) fi lter-sterilized tetrazolium mixture, i.e. 2,3,5-triphenyltetrazolium chloride and tetrazolium blue chloride in the ratio of 9:1, and 1 ml/l of 1 % (w/v) fi lter-sterilized potassium tellurite
were added. When solidifi ed, the medium was amber coloured.
Nutrient Agar Supplemented with Activated Charcoal for Fla.
psychrophilum (Álvarez and Guijarro 2007 )
Nutrient agar supplemented with 0.05 % (w/v) activated charcoal
Dorset-Egg Medium
75 % (v/v) fresh egg mixture (whites and yolks)
0.25 % (w/v) lab-lemco powder
0.25 % (w/v) peptone
0.125 % (w/v) sodium chloride
12 Isolation/Detection
Improved Growth Medium for Fla. psychrophilum (Daskalov
et al. 1999 )
Cytophaga agar/broth supplemented with:
0.05 % (w/v) D(+) galactose
0.05 % (w/v) D(+) glucose
0.05 % (w/v) skimmed milk powder
The supplement were prepared as 10 % (w/v) solutions, and fi ltered separately
through 0.22 μm porosity fi lters, and added to molten, cooled medium
LG Agar for Lactococcus garvieae (Chang et al. 2014 )
5 % (w/v) sucrose
3 % (w/v) oxgall (Difco)
0.9 % (w/v) proteose peptone No. 3
0.6 % (w/v) pancreatic digest of casein
0.5 % (w/v) proteose peptone
0.1 % (w/v) dextrose
1.5 % (w/v) agar
The ingredients were dissolved, adjusted to pH 7.0 before sterilizing at
121 °C/15 min.
The medium was cooled to 55 °C, and 8 ml/l of 1 % (w/v) fi lter-sterilized tetrazolium mixture, i.e. 2,3,5-triphenyltetrazolium chloride and tetrazolium blue chloride in the ratio of 9:1, and 1 ml/l of 1 % (w/v) fi lter-sterilized potassium tellurite
were added. When solidifi ed, the medium was amber coloured.
Nutrient Agar Supplemented with Activated Charcoal for Fla.
psychrophilum (Álvarez and Guijarro 2007 )
Nutrient agar supplemented with 0.05 % (w/v) activated charcoal
Dorset-Egg Medium
75 % (v/v) fresh egg mixture (whites and yolks)
0.25 % (w/v) lab-lemco powder
0.25 % (w/v) peptone
0.125 % (w/v) sodium chloride
12 Isolation/Detection