649
Columbia Agar
2.3 % (w/v) special peptone
0.1 % (w/v) starch
0.5 % (w/v) sodium chloride
1 % (w/v) agar No. 1
pH 7.3
Sterilise at 121 °C/15 min.
Coomassie Brilliant Blue Agar (CBB)
0.01 % (w/v) coomassie brilliant blue dye [C.I. 42655]
TSA
sterilise at 121 °C/15 min.
Cysteine Blood Agar
0.3 % (w/v) beef extract
0.1 % (w/v) L-cysteine hydrochloride
20 % (v/v) human blood
0.5 % (w/v) sodium chloride
0.05 % (w/v) yeast extract
1.5 % (w/v) agar
Cytophaga Agar (Anacker and Ordal 1959 )
0.05 % (w/v) tryptone
0.05 % (w/v) yeast extract
0.02 % (w/v) sodium acetate
0.02 % (w/v) beef extract
0.9 % or 1.0 % (w/v) agar
pH 7.2–7.4; sterilise at 121 °C for 15 min.
Appendix – Media Used for the Isolation and Growth of Bacterial Fish Pathogens