648
0.008 % (w/v) CaCl 2 .2H 2 O
0.002 % (w/v) FeSO 4 .7H 2 O
pH 6.6; 121 °C/15 min – but CaCl 2 .2H 2 O and FeSO 4 .7H 2 O were sterilised separately to avoid precipitation; glucose was also sterilized separately to the yeast
extract and peptone.
Bootsma and Clerx’s Medium for Flavobacterium columnare
0.05 % (w/v) casitone
0.05 % (w/v) yeast extract
1.0 % (w/v) agar
pH 8.0
Brewer’s Thioglycollate Medium
0.1 %(w/v) Lab-lemco
0.2 % (w/v) yeast extract
0.5 % (w/v) peptone
0.5 % (w/v) dextrose
0.5 % (w/v) sodium chloride
0.11 % (w/v) sodium thioglycollate
0.0002 % (w/v) methylene blue
0.1 % (w/v) agar No. 1
pH 7.2
Sterilise at 121 °C/15 min
Charcoal Agar; for the Growth of Renibacterium
salmoninarum
1.0 % (w/v) peptone
0.05 % (w/v) yeast extract
0.1 % (w/v) L-cysteine hydrochloride
0.1 % (w/v) activated charcoal
1.5 % (w/v) agar
pH 6.8; sterilise at 121 °C for 15 min (the charcoal may be placed in dialysis tubing
prior to sterilisation in order to obtain a clear broth medium).
12 Isolation/Detection