654
0.1 % (w/v) calcium chloride
1.5 % (w/v) agar
pH 7.0; sterilise at 121 °C/15 min
Middlebrook 7H10 Agar
0.05 % (w/v) ammonium sulphate
0.15 % (w/v) potassium phosphate
0.15 % (w/v) sodium phosphate
0.04 % (w/v) sodium citrate
0.0025 % (w/v) magnesium sulphate
0.00005 % (w/v) calcium chloride hydrated
0.0001 % (w/v) zinc sulphate
0.0001 % (w/v) cupric sulphate
0.05 % (w/v) L-glutamic acid
0.5 % (v/v) glycerol
0.04 % (w/v) ferric ammonium citrate
0.0001 % (w/v) pyridozine
0.00005 % (w/v) biotin
0.000025 % (w/v) malachite green
1.5 % (w/v) agar
pH 6.6; sterilise at 121 ° C for 15 min, cool to 50–55 °C and add supplements of
either bovine albumin – fraction V, glucose and beef catalase (to 0.5 % w/v,
0.2 % w/v and 0.0003 % w/v, respectively); oleic acid, bovine albumin – fraction
V, glucose, beef catalase and sodium chloride (to 0.005 % v/v, 0.05 % w/v,
0.2 % w/v, 0.0004 % w/v and 0.085 % w/v, respectively); or oleic acid, bovine
albumin – fraction V, glucose, beef catalase, sodium chloride and Triton (to
0.005 % v/v, 0.05 % w/v, 0.02 % w/v, 0.0004 % w/v, 0.085 % w/v and 0.025 % v/v,
respectively).
Myxobacterium Selective Medium (Hsu et al. 1983 )
0.3 % (w/v) casein
0.2 % (w/v) tryptone
0.05 % (w/v) yeast extract
0.03 % (w/v) calcium chloride
1.0 % (w/v) agar
pH 7.0
sterilise at 121 °C/15 min, allow to cool to ~50 °C, and add fi lter sterilised antibiotic
solution:
12 Isolation/Detection