645
Table 12.1 (continued)
Medium
a
Temperature of
incubation (°C )
Pathogen
Cytophaga agar
18–20 °C
Fla. branchiophilum, Fla columnare, Fla.
hydatis, Fla. johnsoniae, Fla. ovolyticus, Fla.
psychrophilus
Cytophaga agar
prepared in sea water
20 °C
Chryseobacterium spp. , Tenacibaculum spp.,
Myxococcus piscicola, Sporocytophaga sp.
(possibly)
Cytophaga agar
supplemented with
5 mg of neomycin/ml
and 200 IU of
polymyxin B/ml
30 °C
Flectobacillus roseus
Fle. maritimus
medium
20 °C
Tenacibaculum spp., Ps. baetica
Glucose asparagine
agar
25–37 °C
Noc. salmonicida
Hayfl ick medium
room temperature
Mycoplasma mobile
Hsu-Shotts medium
22 °C
Fla. spartansii
Hsu-Shotts medium
supplemented with
4 mg of neomycin/ml
22 °C
Chryseobacterium spp.
KDM2/SKDM
15 °C
Ren. salmoninarum
LG agar
25–30 °C
Lactococcus garvieae
Löwenstein-Jensen
medium/Dorset egg
medium
15–22 °C
Mycobacterium spp., Nocardia spp.
Marine 2216E agar
20–30 °C
Myroides odoratimimus, Pseudoalteromonas
piscicida, .
Middlebrook 7H10
agar
30 °C
Mycobacterium spp.
Middlebrook medium 25 °C
Myc. montefi orense
Mueller Hinton agar
supplemented with
5 % (v/v) sheep blood
26 °C
Lactococcus lactis subsp. lactis
Nutrient agar
20–25 °C
Haf. alvei, Ps. anguilliseptica, Ps. chlororaphis,
V. cholerae
Nutrient agar
37 °C
Bacillus spp.
Nutrient agar
supplemented with
0.05 % (w/v) activated
charcoal
20 °C
Fla. psychrophilum
1 % Ogawa-egg
medium
30 °C
Myc. gordonae
0.1 % (w/v) peptone
and agar prepared in
sea water
20–25 °C
Fla. piscicida
(continued)
12 Isolation/Detection