644
Table 12.1 Methods of isolation for bacterial fi sh pathogens
Medium
a
Temperature of
incubation (°C )
Pathogen
Aeromonas selective
medium
?
Aer. veronii biovar sobria
AUSTRAL-SRS
broth + L-cysteine
18 °C
Piscirickettsia salmonis
BHIA
20–37 °C
Aer. schubertii, Aer. sobria, Cit. freundii, Cor.
aquaticum, Delftia acidivorans, Edw. ictaluri,
Edw. tarda, Haf. alvei, Halomonas cupida,
Lactococcus garvieae, Lactococcus piscium,
Planococcus sp., Ps. alcaligenes, Sal. enterica
subsp . arizonae, Ser. liquefaciens, Ser.
marcescens, Sta. aureus, Sta. epidermidis,
Streptococcus spp., Y. ruckeri
BHIA
22–24 °C
(anerobically)
Eu. tarantellae
BHIA supplemented
with 5–10 % (v/v)
blood
20–25 °C
Aerococcus viridans, Cit. freundii, Haf. alvei,
Ph. damselae, Salmonella enterica subsp.
arizonae
BHIA supplemented
with 3 % (w/v) NaCl
15–25 °C
Shewanella putrefaciens
BHIA supplemented
with 10 % (v/v) foetal
calf serum and 1 %
(w/v) NaCl
22 °C
Streptobacillus
Blood agar
15–37 °C
streptococci, Vag. salmoninarum, Y. intermedia
Blood agar
15, 22 or 25 °C
Aer. caviae, Bacillus mycoides, Edw. piscicida,
Erysipelothrix rhusiopathiae Myc. neoaurum,
Rhodococcus erythropolis, Rhodococcus
qingshengii, Weissella sp.
Blood agar
supplemented with
1.5 % (w/v) NaCl
37 °C
Weissella ceti
15 °C
V. splendidus, V. tapetis
Blood agar
supplemented with
0.5–1.5 % (w/v) NaCl
22–25 °C
Acinetobacter sp., Ph. damselae subsp.
piscicida, Ali. logei, Moritella marina,
Pasteurella skyensis
Campylobacter -
selective agar
25 °C
Arcobacter cryaerophilus
CBB
15–25 °C
Aer. salmonicida
Cresol red thallium
acetate sucrose inulin
agar
22 °C
Carnobacterium maltaromaticum -like organ
Cystine heart agar
supplemented with
1 % (w/v)
haemoglobin
?
Francisella sp.
(continued)
12 Isolation/Detection
Table 12.1 Methods of isolation for bacterial fi sh pathogens
Medium
a
Temperature of
incubation (°C )
Pathogen
Aeromonas selective
medium
?
Aer. veronii biovar sobria
AUSTRAL-SRS
broth + L-cysteine
18 °C
Piscirickettsia salmonis
BHIA
20–37 °C
Aer. schubertii, Aer. sobria, Cit. freundii, Cor.
aquaticum, Delftia acidivorans, Edw. ictaluri,
Edw. tarda, Haf. alvei, Halomonas cupida,
Lactococcus garvieae, Lactococcus piscium,
Planococcus sp., Ps. alcaligenes, Sal. enterica
subsp . arizonae, Ser. liquefaciens, Ser.
marcescens, Sta. aureus, Sta. epidermidis,
Streptococcus spp., Y. ruckeri
BHIA
22–24 °C
(anerobically)
Eu. tarantellae
BHIA supplemented
with 5–10 % (v/v)
blood
20–25 °C
Aerococcus viridans, Cit. freundii, Haf. alvei,
Ph. damselae, Salmonella enterica subsp.
arizonae
BHIA supplemented
with 3 % (w/v) NaCl
15–25 °C
Shewanella putrefaciens
BHIA supplemented
with 10 % (v/v) foetal
calf serum and 1 %
(w/v) NaCl
22 °C
Streptobacillus
Blood agar
15–37 °C
streptococci, Vag. salmoninarum, Y. intermedia
Blood agar
15, 22 or 25 °C
Aer. caviae, Bacillus mycoides, Edw. piscicida,
Erysipelothrix rhusiopathiae Myc. neoaurum,
Rhodococcus erythropolis, Rhodococcus
qingshengii, Weissella sp.
Blood agar
supplemented with
1.5 % (w/v) NaCl
37 °C
Weissella ceti
15 °C
V. splendidus, V. tapetis
Blood agar
supplemented with
0.5–1.5 % (w/v) NaCl
22–25 °C
Acinetobacter sp., Ph. damselae subsp.
piscicida, Ali. logei, Moritella marina,
Pasteurella skyensis
Campylobacter -
selective agar
25 °C
Arcobacter cryaerophilus
CBB
15–25 °C
Aer. salmonicida
Cresol red thallium
acetate sucrose inulin
agar
22 °C
Carnobacterium maltaromaticum -like organ
Cystine heart agar
supplemented with
1 % (w/v)
haemoglobin
?
Francisella sp.
(continued)
12 Isolation/Detection