646
Table 12.1 (continued)
Medium
a
Temperature of
incubation (°C )
Pathogen
Robertson’s meat
broth
30 °C
(anaerobically)
Cl. botulinum
Seawater agar (marine
2216E agar)
15–25 °C
Ph. damselae subsp. piscicida, Ali. fi scheri,
Vibrio spp., Pseudoalteromonas undina
Skimmed milk agar
15–25 °C
Janthinobacterium sp., Micrococcus luteus,
Planococcus sp.
TCBS
15–25 °C
Delftia acidivorans, Ph. damselae, Ali. fi scheri V.
alginolyticus, V. anguillarum,, V. harveyi, V.
ordalii, V. pelagius and V. splendidus
Tissue culture
(salmonid cell line)
12–21 °C
Francisella sp., Piscirickettsia salmonis
Todd-Hewitt agar
supplemented with
30 μg/ml of Congo red
37 °C for 30 h
Str. dysgalactiae
TSA [possibly
supplemented with
1–2 % (w/v) NaCl
15–25 °C
Aer. allosaccharophila, Aer. caviae, Aer.
dhakensis, Aer. hydrophila, Aer. piscicola. Aer.
salmonicida, Aer. sobria, Bacillus sp ., Car.
piscicola, Cit. freundii, Delftia acidivorans, Edw.
piscicida, Enterobacter cloacae, En. faecalis
subsp. liquefaciens, Esch. vulneris, Pantoea
agglomerans, Providencia vermicola,
Flavobacterium sp., Hahella chejuensis, J.
lividum, Klebsiella pneumoniae, Lactobacillus
spp., Lactococcus lactis subsp. lactis,
Micrococcus luteus, Moraxella sp., Planococcus
sp., Plesiomonas shigelloides, Pr. rettgeri,
Pseudomonas spp. , Rhodococcus sp.,
Rhodococcus qingshengii, Ser. marcescens, Ser.
plymuthica, Sta. warneri, Ali. salmonicida, V.
harveyi, V. pelagius, V. splendidus, V. vulnifi cus,
Y. ruckeri
TSA supplemented
with 10 % (v/v) horse
serum
unspecifi ed
Aquaspirillum
TSA supplemented
with 5 % (v/v)
defi brinated sheep
blood
30 °C
Str. ictaluri
Yeast extract glucose
agar
25 °C
Vag. salmoninarum
a Most of these media may be obtained from Difco and/or Oxoid
12 Isolation/Detection
Table 12.1 (continued)
Medium
a
Temperature of
incubation (°C )
Pathogen
Robertson’s meat
broth
30 °C
(anaerobically)
Cl. botulinum
Seawater agar (marine
2216E agar)
15–25 °C
Ph. damselae subsp. piscicida, Ali. fi scheri,
Vibrio spp., Pseudoalteromonas undina
Skimmed milk agar
15–25 °C
Janthinobacterium sp., Micrococcus luteus,
Planococcus sp.
TCBS
15–25 °C
Delftia acidivorans, Ph. damselae, Ali. fi scheri V.
alginolyticus, V. anguillarum,, V. harveyi, V.
ordalii, V. pelagius and V. splendidus
Tissue culture
(salmonid cell line)
12–21 °C
Francisella sp., Piscirickettsia salmonis
Todd-Hewitt agar
supplemented with
30 μg/ml of Congo red
37 °C for 30 h
Str. dysgalactiae
TSA [possibly
supplemented with
1–2 % (w/v) NaCl
15–25 °C
Aer. allosaccharophila, Aer. caviae, Aer.
dhakensis, Aer. hydrophila, Aer. piscicola. Aer.
salmonicida, Aer. sobria, Bacillus sp ., Car.
piscicola, Cit. freundii, Delftia acidivorans, Edw.
piscicida, Enterobacter cloacae, En. faecalis
subsp. liquefaciens, Esch. vulneris, Pantoea
agglomerans, Providencia vermicola,
Flavobacterium sp., Hahella chejuensis, J.
lividum, Klebsiella pneumoniae, Lactobacillus
spp., Lactococcus lactis subsp. lactis,
Micrococcus luteus, Moraxella sp., Planococcus
sp., Plesiomonas shigelloides, Pr. rettgeri,
Pseudomonas spp. , Rhodococcus sp.,
Rhodococcus qingshengii, Ser. marcescens, Ser.
plymuthica, Sta. warneri, Ali. salmonicida, V.
harveyi, V. pelagius, V. splendidus, V. vulnifi cus,
Y. ruckeri
TSA supplemented
with 10 % (v/v) horse
serum
unspecifi ed
Aquaspirillum
TSA supplemented
with 5 % (v/v)
defi brinated sheep
blood
30 °C
Str. ictaluri
Yeast extract glucose
agar
25 °C
Vag. salmoninarum
a Most of these media may be obtained from Difco and/or Oxoid
12 Isolation/Detection