560
Vibrio pelagius
Characteristics of the Disease
An epizootic of juvenile farmed turbot in Northwest Spain occurred during January
and February 1991 when the water temperature was 12–15 °C, with fi sh displaying
eroded dorsal fi ns and tail, haemorrhages at the base if the fi ns, haemorrhages on the
internal organs, and intestines full of mucus liquid (Angulo et al. 1992 ). The total
losses amounted to 3 % of the turbot population. Subsequently, larval turbot were
described with swollen and necrotic secondary gill lamellae, sloughing-off of the
intestinal mucosa, and necrosis of the haematopoietic tissues of the kidney (Villamil
et al. 2003a ).
Isolation
Samples from diseased tissues were inoculated onto TSA supplemented with 2 %
(w/v) sodium chloride, marine 2216E agar and TCBS with incubation at 25 °C for
48 h (Angulo et al. 1992 ).
Characteristics of the Pathogen
Four isolates were obtained in pure culture, and described as (Angulo et al. 1992 ):
Box 10.14: Vibrio pelagius
The cultures comprise Gram-negative motile fermentative rods, that produce
catalase, ß-galactosidase (variable), indole (variable) and oxidase, but not
arginine dihydrolase, H 2 S, or lysine or ornithine decarboxylase, reduce
nitrates, degrade alginate (variable), gelatin, starch, Tween 80 and urea (variable), produce acid from mannitol, mannose, trehalose and sucrose (variable)
but not L-arabinose, arbutin, inositol, salicin or sorbitol, and are sensitive to
the vibriostatic agent O/129, ampicillin and novobiocin. The methyl red test
is generally positive. The Voges Proskauer reaction gives a variable response.
Growth occurs in 6 % (w/v) but not 0 % or 10 % (w/v) sodium chloride, at
35 °C but not 4 or 42 °C. However, the isolates do not agglutinate with O
antigens of V. pelagius ATCC 25916.
10 Vibrios
Vibrio pelagius
Characteristics of the Disease
An epizootic of juvenile farmed turbot in Northwest Spain occurred during January
and February 1991 when the water temperature was 12–15 °C, with fi sh displaying
eroded dorsal fi ns and tail, haemorrhages at the base if the fi ns, haemorrhages on the
internal organs, and intestines full of mucus liquid (Angulo et al. 1992 ). The total
losses amounted to 3 % of the turbot population. Subsequently, larval turbot were
described with swollen and necrotic secondary gill lamellae, sloughing-off of the
intestinal mucosa, and necrosis of the haematopoietic tissues of the kidney (Villamil
et al. 2003a ).
Isolation
Samples from diseased tissues were inoculated onto TSA supplemented with 2 %
(w/v) sodium chloride, marine 2216E agar and TCBS with incubation at 25 °C for
48 h (Angulo et al. 1992 ).
Characteristics of the Pathogen
Four isolates were obtained in pure culture, and described as (Angulo et al. 1992 ):
Box 10.14: Vibrio pelagius
The cultures comprise Gram-negative motile fermentative rods, that produce
catalase, ß-galactosidase (variable), indole (variable) and oxidase, but not
arginine dihydrolase, H 2 S, or lysine or ornithine decarboxylase, reduce
nitrates, degrade alginate (variable), gelatin, starch, Tween 80 and urea (variable), produce acid from mannitol, mannose, trehalose and sucrose (variable)
but not L-arabinose, arbutin, inositol, salicin or sorbitol, and are sensitive to
the vibriostatic agent O/129, ampicillin and novobiocin. The methyl red test
is generally positive. The Voges Proskauer reaction gives a variable response.
Growth occurs in 6 % (w/v) but not 0 % or 10 % (w/v) sodium chloride, at
35 °C but not 4 or 42 °C. However, the isolates do not agglutinate with O
antigens of V. pelagius ATCC 25916.
10 Vibrios