441
Pathogenicity
The descriptions of T. discolor contained a statement that pathogenicity had been
achieved in turbot and sole (Piñeiro-Vidal et al. 2007 , 2008c ).
Tenacibaculum gallaicum
Characteristics of the Disease
Diseased fi sh were described as displaying eroded mouth, rotted fi ns, shallow skin
lesions and pale internal organs (Piñeiro-Vidal et al. 2008c ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c ; 2012 ).
Characteristics of the Pathogen
A single isolate was recovered from diseased turbot in Spain:
Box 7.18: Tenacibaculum gallaicum
Cultures comprise fl at bright yellow-pigmented strictly aerobic Gramnegative rods of 2–30 × 0.5 μm in size, which demonstrate gliding movement.
Older cells may become spherical. Growth occurs at 14–38 °C (optimally at
25–30 °C) and pH 6.0–8.0, and in media containing 30–100 % seawater but
not in media supplemented only with sodium chloride. Catalase and oxidase
are produced, but not H 2 S or indole. Acid and alkaline phosphatase,
α-chymotrypsin, esterase, esterase lipase, lipase, cystine, leucine and valine
arylamidases, naphthol-AS-BI-phosphohydrolase and trypsin are produced.
Nitrates are reduced. Casein and gelatin are degraded, but not starch or Tween
80. The Voges Proskauer reaction is negative. Acid is not produced from carbohydrates. L-proline and L-glutamate are utilized, but not D-galactose,
D-glucose, D-ribose, D-sucrose or L-tyrosine. The G + C ratio of the DNA
was reported as 32.7 moles % (Piñeiro-Vidal et al. 2008a ).
Tenacibaculum gallaicum
Pathogenicity
The descriptions of T. discolor contained a statement that pathogenicity had been
achieved in turbot and sole (Piñeiro-Vidal et al. 2007 , 2008c ).
Tenacibaculum gallaicum
Characteristics of the Disease
Diseased fi sh were described as displaying eroded mouth, rotted fi ns, shallow skin
lesions and pale internal organs (Piñeiro-Vidal et al. 2008c ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c ; 2012 ).
Characteristics of the Pathogen
A single isolate was recovered from diseased turbot in Spain:
Box 7.18: Tenacibaculum gallaicum
Cultures comprise fl at bright yellow-pigmented strictly aerobic Gramnegative rods of 2–30 × 0.5 μm in size, which demonstrate gliding movement.
Older cells may become spherical. Growth occurs at 14–38 °C (optimally at
25–30 °C) and pH 6.0–8.0, and in media containing 30–100 % seawater but
not in media supplemented only with sodium chloride. Catalase and oxidase
are produced, but not H 2 S or indole. Acid and alkaline phosphatase,
α-chymotrypsin, esterase, esterase lipase, lipase, cystine, leucine and valine
arylamidases, naphthol-AS-BI-phosphohydrolase and trypsin are produced.
Nitrates are reduced. Casein and gelatin are degraded, but not starch or Tween
80. The Voges Proskauer reaction is negative. Acid is not produced from carbohydrates. L-proline and L-glutamate are utilized, but not D-galactose,
D-glucose, D-ribose, D-sucrose or L-tyrosine. The G + C ratio of the DNA
was reported as 32.7 moles % (Piñeiro-Vidal et al. 2008a ).
Tenacibaculum gallaicum