440
Tenacibaculum discolor
Characteristics of the Disease
Diseased fi sh were described as displaying eroded mouth, rotted fi ns, shallow skin
lesions and pale internal organs (Piñeiro-Vidal et al. 2008c ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c ; 2012 ).
Characteristics of the Pathogen
A single isolate was recovered from diseased sole in Spain:
By sequencing the 16S rRNA gene, the closest neighbour was T. litoreum
(homology = 99.4 %) ; the corresponding DNA:DNA hybridization value was 39.6 %
(Piñeiro-Vidal et al. 2008c ).
Box 7.17: Tenacibaculum discolor
Cultures comprise fl at bright yellow-pigmented [not fl exirubin] strictly aerobic straight Gram-negative rods of 2–30 × 0.5 μm in size, which demonstrate
gliding movement. Older cells may become spherical. Growth occurs at
14–38 °C (optimally at 25–30 °C) and pH 6.0–8.0, and in media containing
30–100 % seawater but not in media supplemented only with sodium chloride.
Catalase and oxidase are produced, but not H 2 S or indole. Acid and alkaline
phosphatase, α-chymotrypsin, esterase, esterase lipase, lipase, cystine, leucine and valine arylamidases, naphthol-AS-BI-phosphohydrolase and trypsin
are produced. Nitrates are reduced. Casein and gelatin are degraded, but not
starch or Tween 80. The Voges Proskauer reaction is negative. Acid is not
produced from carbohydrates. L-proline and L-glutamate are utilized, but not
D-galactose, D-glucose, D-ribose, D-sucrose or L-tyrosine. The G + C ratio of
the DNA was reported as 32.1 moles % (Piñeiro-Vidal et al. 2008c ).
7 Flavobacteria and Cytophagas
Tenacibaculum discolor
Characteristics of the Disease
Diseased fi sh were described as displaying eroded mouth, rotted fi ns, shallow skin
lesions and pale internal organs (Piñeiro-Vidal et al. 2008c ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c ; 2012 ).
Characteristics of the Pathogen
A single isolate was recovered from diseased sole in Spain:
By sequencing the 16S rRNA gene, the closest neighbour was T. litoreum
(homology = 99.4 %) ; the corresponding DNA:DNA hybridization value was 39.6 %
(Piñeiro-Vidal et al. 2008c ).
Box 7.17: Tenacibaculum discolor
Cultures comprise fl at bright yellow-pigmented [not fl exirubin] strictly aerobic straight Gram-negative rods of 2–30 × 0.5 μm in size, which demonstrate
gliding movement. Older cells may become spherical. Growth occurs at
14–38 °C (optimally at 25–30 °C) and pH 6.0–8.0, and in media containing
30–100 % seawater but not in media supplemented only with sodium chloride.
Catalase and oxidase are produced, but not H 2 S or indole. Acid and alkaline
phosphatase, α-chymotrypsin, esterase, esterase lipase, lipase, cystine, leucine and valine arylamidases, naphthol-AS-BI-phosphohydrolase and trypsin
are produced. Nitrates are reduced. Casein and gelatin are degraded, but not
starch or Tween 80. The Voges Proskauer reaction is negative. Acid is not
produced from carbohydrates. L-proline and L-glutamate are utilized, but not
D-galactose, D-glucose, D-ribose, D-sucrose or L-tyrosine. The G + C ratio of
the DNA was reported as 32.1 moles % (Piñeiro-Vidal et al. 2008c ).
7 Flavobacteria and Cytophagas