439
Tenacibaculum dicentrarchi
Characteristics of the Disease
Although recovered from diseased sea bass and proposed as new species, the authors
did not prove that the organism was actually pathogenic in laboratory-based infectivity experiments (Piñeiro-Vidal et al. 2012 ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c , 2012 ).
Characteristics of the Pathogen
The culture was linked to Tenacibaculum as a result of sequencing the 16S rRNA
gene; the homology of 93.1–97.3 % indicating a new species (Piñeiro-Vidal et al.
2012 ).
Box 7.16: Tenacibaculum dicentrarchi
Cultures comprise fl at pale yellow-pigmented [not fl exirubin] strictly aerobic
straight Gram-negative rods of 2–40 [fi laments of up to 150 μm in length may
occur] × 0.3–0.5 μm in size, which demonstrate gliding movement. Older cells
may become spherical. Growth occurs at 4–30 °C and pH 6.0–9.0, and in
media containing 30–100 % seawater [optimally 70 %] but not in media supplemented only with sodium chloride. Catalase, oxidase, alkaline phosphatase, esterase (C4), esterase lipase (C8), lipase (C4), cystine leucine and valine
arylamidases are produced, but not α–chymotrypsin, H 2 S, indole or trypsin.
Casein and gelatin are degraded, but not starch or Tween 80. The Voges
Proskauer reaction is negative. Acid is not produced from carbohydrates.
Susceptible to O/129. The G + C ratio of the DNA was reported as 31.3 moles
% (Piñeiro-Vidal et al. 2012 ).
Tenacibaculum dicentrarchi
Tenacibaculum dicentrarchi
Characteristics of the Disease
Although recovered from diseased sea bass and proposed as new species, the authors
did not prove that the organism was actually pathogenic in laboratory-based infectivity experiments (Piñeiro-Vidal et al. 2012 ).
Isolation
Recovery was achieved on Fle. maritimus medium following incubation at 25 °C
for 48 h (Piñeiro-Vidal et al. 2007 , 2008c , 2012 ).
Characteristics of the Pathogen
The culture was linked to Tenacibaculum as a result of sequencing the 16S rRNA
gene; the homology of 93.1–97.3 % indicating a new species (Piñeiro-Vidal et al.
2012 ).
Box 7.16: Tenacibaculum dicentrarchi
Cultures comprise fl at pale yellow-pigmented [not fl exirubin] strictly aerobic
straight Gram-negative rods of 2–40 [fi laments of up to 150 μm in length may
occur] × 0.3–0.5 μm in size, which demonstrate gliding movement. Older cells
may become spherical. Growth occurs at 4–30 °C and pH 6.0–9.0, and in
media containing 30–100 % seawater [optimally 70 %] but not in media supplemented only with sodium chloride. Catalase, oxidase, alkaline phosphatase, esterase (C4), esterase lipase (C8), lipase (C4), cystine leucine and valine
arylamidases are produced, but not α–chymotrypsin, H 2 S, indole or trypsin.
Casein and gelatin are degraded, but not starch or Tween 80. The Voges
Proskauer reaction is negative. Acid is not produced from carbohydrates.
Susceptible to O/129. The G + C ratio of the DNA was reported as 31.3 moles
% (Piñeiro-Vidal et al. 2012 ).
Tenacibaculum dicentrarchi