325
Disease Control
Disinfection Outbreaks of the disease abated following adoption of water disinfection (with chlorine) practices (Sato et al. 1982 ).
Antimicrobial Compounds
According to Sato et al. ( 1982 ) and Baya et al. ( 1990 ), isolates were resistant to
chloramphenicol, potentiated sulphonamides and tetracycline. Indeed, chemotherapy was unsuccessful at controlling the disease in the Japanese aquarium.
Edwardsiella anguillarum
Cultures were obtained from diseased eels, sea bream and red sea bream in China
(Shao et al. 2015 ).
Characteristics of the Pathogen
Pathogenicity
Pathogenicity was demonstrated in a laboratory infectivity experiment using turbot
in which the LD 50 dose was reported as 5.7 × 10
2 CFU/g (of fi sh). Three sets of distinct T6SSs and 2 T3SSs were present in the type strain (Shao et al. 2015 ).
Box 6.2: Edwardsiella anguillarum
Cultures comprise non-pigmented colonies with irregular margins that contain Gram-negative facultatively anaerobic rods that are motile by peritrichous fl agella. Growth occurs at 18–37 °C (optimally at 28–30 °C) but not
12 °C, in 1–3 % (w/v) sodium chloride, and at pH 6–9 (optimally at pH 7–8).
Catalase, H 2 S and indole are produced, but not arginine dihydrolase,
ß- galactosidase, oxidase or tryptophan deaminase. Neither gelatin nor urea is
degraded. The principal fatty acids of the type strain are C 14:0 , C 16:0 , C 17:0
cyclo, summed feature 2 (C 14:0 3OH/iso-C 16:1 I), summed feature 3 (C 16:1 ω6c/
ω7c) and summed feature 8 (C 16:1 ω6c/ω7c). The respiratory quinone of the
same culture is menaquinone MK-8; also present is phosphatidylethanolamine, phosphatidylglycerol, aminolipid, phospholipid and one unknown
lipid. The G + C ratio of the type strain 58.72 mol% (Shao et al. 2015 ).
Edwardsiella anguillarum
Disease Control
Disinfection Outbreaks of the disease abated following adoption of water disinfection (with chlorine) practices (Sato et al. 1982 ).
Antimicrobial Compounds
According to Sato et al. ( 1982 ) and Baya et al. ( 1990 ), isolates were resistant to
chloramphenicol, potentiated sulphonamides and tetracycline. Indeed, chemotherapy was unsuccessful at controlling the disease in the Japanese aquarium.
Edwardsiella anguillarum
Cultures were obtained from diseased eels, sea bream and red sea bream in China
(Shao et al. 2015 ).
Characteristics of the Pathogen
Pathogenicity
Pathogenicity was demonstrated in a laboratory infectivity experiment using turbot
in which the LD 50 dose was reported as 5.7 × 10
2 CFU/g (of fi sh). Three sets of distinct T6SSs and 2 T3SSs were present in the type strain (Shao et al. 2015 ).
Box 6.2: Edwardsiella anguillarum
Cultures comprise non-pigmented colonies with irregular margins that contain Gram-negative facultatively anaerobic rods that are motile by peritrichous fl agella. Growth occurs at 18–37 °C (optimally at 28–30 °C) but not
12 °C, in 1–3 % (w/v) sodium chloride, and at pH 6–9 (optimally at pH 7–8).
Catalase, H 2 S and indole are produced, but not arginine dihydrolase,
ß- galactosidase, oxidase or tryptophan deaminase. Neither gelatin nor urea is
degraded. The principal fatty acids of the type strain are C 14:0 , C 16:0 , C 17:0
cyclo, summed feature 2 (C 14:0 3OH/iso-C 16:1 I), summed feature 3 (C 16:1 ω6c/
ω7c) and summed feature 8 (C 16:1 ω6c/ω7c). The respiratory quinone of the
same culture is menaquinone MK-8; also present is phosphatidylethanolamine, phosphatidylglycerol, aminolipid, phospholipid and one unknown
lipid. The G + C ratio of the type strain 58.72 mol% (Shao et al. 2015 ).
Edwardsiella anguillarum