1 Genomics in the Discovery and Monitoring
19
Table 1.1 (continued)
Molecular
approach
Organism
targeted
Tested on
larvae?
Used in
ecological
studies?
Hosoi et al.
(2004)
D1/D2/D3 PCR
and RFLP
Barbatia (Abarbatia) virescens,
Mytilus galloprovincialis,
Pinctada martensii, Pinna
bicolor, Chlamys (Azumapecten)
farreri nipponensis, Anomia
chinensis, Crassostrea gigas,
Fuluvia mutica, Lasaea
undulata, Moerella jedoensis,
Theora fragilis, Ruditapes
philippinarum, Paphia undulata
(bivalves)
Yes
No
von der
Heyden
et al.
(2007)
Control region
PCR and
sequencing
Merluccius capensis, M.
paradoxus (Cape hakes)
Yes
No
Richardson
et al.
(2007)
Cyt b PCR and
sequencing
Istiophorus platypterus, Makaira
nigricans, Tetrapturus albidus,
T. pfluegeri (billfish), Thunnus
atlanticus, T. albacares, T.
thynnus, T. obsesus, T. alalunga
(tuna)
Yes
Yes
Webb et al.
(2006)
COI, 16S and 18S
PCR and
sequencing
Solasteridae, Echinidae,
Ophiuridae, Echinidae, Syllida,
Serpulidae, Nudibranchia,
Gastropoda, Nemertea,
Urochordata, Ophiuroidea,
Terebellidea, Adamussium
colbecki, (Antarctic scallop)
Parborlasia corrugatus, (Antarctic
nemertean)
Yes
No
Kirby and
Lindley
(2005)
16 S nested PCR
and sequencing
Echinocardium cordatum,
Marthasterias glacialis,
Spatangus purpureus, Amphiura
filiformis (echinoderms)
Yes
Yes
Barber and
Boyce
(2006)
COI PCR and
sequencing
Stomatopod larvae (scleractinian
corals)
Yes
Yes
Goffredi et al.
(2006)
18S PCR and
sandwich
hybridisation
Thoracica, Balanus glandula
(barnacles)
Yes
Yes
Jones et al.
(2008)
18S PCR and
sandwich
hybridisation
Carcinus maenas (green crab),
Mytilus edulis (native blue
mussel), Balanus sp. (barnacle),
Osedax sp. and Ophelia sp.
(polychaetes)
Yes
Yes
Deagle et al.
(2003)
PCR and DGGE Asterias amurensis (northern
Pacific seastar)
Yes
No