Contents XIX
Part B Tools and Methods in Marine Biotechnology
9 Bioprocess Engineering of Phototrophic Marine Organisms
Gregory L. Rorrer.................................................................
257
9.1
Introduction to Marine Process Engineering ..........................
257
9.2
Growth Characteristics of Phototrophic Suspension Cultures ........
261
9.3
Basic Elements of Photobioreactor Design and Operation ...........
267
9.4
Limiting Factors in Photobioreactor Design and Operation ..........
277
9.5
Future Directions for Process Scale Enclosed Photobioreactors ......
290
9.6
Notation..................................................................
291
References .......................................................................
293
10 Bioinformatic Techniques on Marine Genomics
A. Mir Bilal, H. Mir Sajjad, Inho Choi, Yoon-Bo Shim ........................
295
10.1 Background ..............................................................
295
10.2 Marine and Bacterial Fluorescence Shining Light
on Biological Questions .................................................
297
10.3 Recent Advances in Imaging Techniques for Marine Biotechnology
297
10.4 Chemical Analysis of Volatile Microbial Metabolites ..................
297
10.5 Bioinformatics Resources ...............................................
298
10.6 Large-Scale Sequence Analysis .........................................
299
10.7 Integrating Sequence and Contextual Data ...........................
300
10.8 Proteomics as Potential Tool for Survey in Marine Biotechnology...
301
10.9 Proteomics and Seafood ................................................
301
10.10 Present Status and Future Trends of Proteomics
in Marine Biotechnology................................................
302
10.11 Pharmacophore Model Hypo1, Virtual Screening for Identification
of Novel Tubulin Inhibitors with Potent Anticancer Activity .........
302
10.12 The Polymerase Chain Reaction: A Marine Perspective ...............
303
10.13 Conclusions and New Frontiers.........................................
303
References .......................................................................
304
11 Microbial Bioprospecting in Marine Environments
Mariana Lozada, Hebe M. Dionisi ..............................................
307
11.1 Bioprospecting...........................................................
307
11.2 Marine Microbial Habitats and Their Biotechnologically-Relevant
Microorganisms ..........................................................
308
11.3 Methods for Microbial Bioprospecting in Marine Environments.....
309
11.4 Conclusions ..............................................................
319
References .......................................................................
319
12 Novel Bioreactors for Culturing Marine Organisms
Debashis Roy.....................................................................
327
12.1 Biofilm Reactors (BFR)...................................................
327
12.2 Photobioreactors (PBR)-Tubular, Plate/Panel
and Stirred Tank Configurations ........................................
331
12.3 Airlift Bioreactors (ALBR) and Bubble Column Bioreactors (BCBR) ...
337
12.4 Membrane Bioreactors (MBR) ...........................................
349
Part B Tools and Methods in Marine Biotechnology
9 Bioprocess Engineering of Phototrophic Marine Organisms
Gregory L. Rorrer.................................................................
257
9.1
Introduction to Marine Process Engineering ..........................
257
9.2
Growth Characteristics of Phototrophic Suspension Cultures ........
261
9.3
Basic Elements of Photobioreactor Design and Operation ...........
267
9.4
Limiting Factors in Photobioreactor Design and Operation ..........
277
9.5
Future Directions for Process Scale Enclosed Photobioreactors ......
290
9.6
Notation..................................................................
291
References .......................................................................
293
10 Bioinformatic Techniques on Marine Genomics
A. Mir Bilal, H. Mir Sajjad, Inho Choi, Yoon-Bo Shim ........................
295
10.1 Background ..............................................................
295
10.2 Marine and Bacterial Fluorescence Shining Light
on Biological Questions .................................................
297
10.3 Recent Advances in Imaging Techniques for Marine Biotechnology
297
10.4 Chemical Analysis of Volatile Microbial Metabolites ..................
297
10.5 Bioinformatics Resources ...............................................
298
10.6 Large-Scale Sequence Analysis .........................................
299
10.7 Integrating Sequence and Contextual Data ...........................
300
10.8 Proteomics as Potential Tool for Survey in Marine Biotechnology...
301
10.9 Proteomics and Seafood ................................................
301
10.10 Present Status and Future Trends of Proteomics
in Marine Biotechnology................................................
302
10.11 Pharmacophore Model Hypo1, Virtual Screening for Identification
of Novel Tubulin Inhibitors with Potent Anticancer Activity .........
302
10.12 The Polymerase Chain Reaction: A Marine Perspective ...............
303
10.13 Conclusions and New Frontiers.........................................
303
References .......................................................................
304
11 Microbial Bioprospecting in Marine Environments
Mariana Lozada, Hebe M. Dionisi ..............................................
307
11.1 Bioprospecting...........................................................
307
11.2 Marine Microbial Habitats and Their Biotechnologically-Relevant
Microorganisms ..........................................................
308
11.3 Methods for Microbial Bioprospecting in Marine Environments.....
309
11.4 Conclusions ..............................................................
319
References .......................................................................
319
12 Novel Bioreactors for Culturing Marine Organisms
Debashis Roy.....................................................................
327
12.1 Biofilm Reactors (BFR)...................................................
327
12.2 Photobioreactors (PBR)-Tubular, Plate/Panel
and Stirred Tank Configurations ........................................
331
12.3 Airlift Bioreactors (ALBR) and Bubble Column Bioreactors (BCBR) ...
337
12.4 Membrane Bioreactors (MBR) ...........................................
349