Applications in Genetics
Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach
STEFAN FRONHOFFS, THOMAS BRUNING, HANS VETTER, YON Ko
Detection of a Single Base Substitution in Single Cells
by Melting Peak Analysis Using Dual-Color Hybridization Probes
GERARD PALS
Rapid Screening for Five Major Cystic Fibrosis Mutations
by Melting Peak Analysis Using Fluorogenic Hybridization Probes
SIEGFRIED BURGGRAF, NAEEM MALIK,
EDITH SCHUHMACHER, BERNHARD OLGEMOLLER
LightCycier PCR for the Polymorphisms -308 and -238 in the TNF Alpha Gene
67
77
85
and for the TNFB1/B2 Polymorphism in the LT Alpha Gene . . . . . . . . . . . . . . . . . . .
95
LUKAS BESTMANN, NADER HELMY, FELICIA GAROFALO,
AYNUR DEMIRTAS, DIETER VONDERSCHMITT, FRIEDRICH E. MALY
Rapid Genotyping of 2-bp and 9-bp Deletion Mutations
Using the LightCycier Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 107
TsuToMu AOSHIMA, MITSUHARU KAJITA, YOSHITAKA SEKIDO,
SHUNJI MIMURA, KAZUYOSHI WATANABE, KAORU SHIMOKATA,
TOSHIMITSU NIWA
Genotyping ofthe Methionine-Valine Polymorphism at Codon 129
of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled
Hybridization Probes .............................................. 115
SIEGFRIED BURGGRAF, SIEGFRIED KOSEL, SABINE LOHMANN,
REINHARD BECK, BERNHARD OLGEMOLLER
Rapid Detection of Missense Mutations in the Prostatic Steroid
Sa-Reductase Gene Using Real-Time Fluorescence PCR
and Melting Curve Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 129
MARKUS NAUCK, WINFRIED MARZ, HEINRICH WIELAND
II