SYBR Green 1 AnaJysis of the Trinucleotide Repeat Responsible for Huntington's Disease n
ferent from the protocol in table 4 in that the target temperature in segment 2 is
increased to 72°C to prevent primer extension during each melting curve.
In contrast, the control software of the ABI 5700 has a fixed melting protocol.
With LightCycler, the user can also alter the temperature interval averaged when
calculating derivative melting curves. If the interval is too large, separable peaks
converge and sensitivity is lost (Fig. 5).
References
A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's
disease chromosomes. (1993). The Huntington's Disease Collaborative Research Group. Cell.
72( 6):971-83.
Andrew SE, Goldberg YP, Theilmann J, Zeisler J, Hayden MR. (1994). A CCG repeat polymorphism adjacent to the CAG repeat in the Huntington disease gene: implications for diagnostic accuracy and predictive testing. Hum Mol Genet. 3(1):65-7.
Bernard PS, Pritham GH, Wittwer CT. (1999). Color multiplexing hybridization probes using the
apolipoprotein E locus as a model system for genotyping. AnaJ Biochem. 273(2):221-8.
Kremer B, Goldberg P, Andrew SE, Theilmann J, Telenius H, Zeisler J, Squitieri F, Lin B, Bassett
A, Almqvist E, et aJ. (1994). A worldwide study of the Huntington's disease mutation. The
sensitivity and specificity of measuring CAG repeats. N Engl J Med. 330(20):1401-6.
Rubinsztein DC, Leggo J, Barton DE, Ferguson-Smith MA. (1993). Site of (CCG) polymorphism
in the HD gene. Nat Genet. 5(3):214-5.
Thomas SM, Moreno RF, Tilzer L1. (1989). DNA extraction with organic solvents in gel barrier
tubes. Nucleic Acids Res. 17(13):5411.
Wittwer CT, Marshall BC, Reed GH, Cherry J1. (1993). Rapid cycle aJlele-specific amplification:
studies with the cystic fibrosis delta F50810cus. Clin Chern. 39(5):804-9.
ferent from the protocol in table 4 in that the target temperature in segment 2 is
increased to 72°C to prevent primer extension during each melting curve.
In contrast, the control software of the ABI 5700 has a fixed melting protocol.
With LightCycler, the user can also alter the temperature interval averaged when
calculating derivative melting curves. If the interval is too large, separable peaks
converge and sensitivity is lost (Fig. 5).
References
A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's
disease chromosomes. (1993). The Huntington's Disease Collaborative Research Group. Cell.
72( 6):971-83.
Andrew SE, Goldberg YP, Theilmann J, Zeisler J, Hayden MR. (1994). A CCG repeat polymorphism adjacent to the CAG repeat in the Huntington disease gene: implications for diagnostic accuracy and predictive testing. Hum Mol Genet. 3(1):65-7.
Bernard PS, Pritham GH, Wittwer CT. (1999). Color multiplexing hybridization probes using the
apolipoprotein E locus as a model system for genotyping. AnaJ Biochem. 273(2):221-8.
Kremer B, Goldberg P, Andrew SE, Theilmann J, Telenius H, Zeisler J, Squitieri F, Lin B, Bassett
A, Almqvist E, et aJ. (1994). A worldwide study of the Huntington's disease mutation. The
sensitivity and specificity of measuring CAG repeats. N Engl J Med. 330(20):1401-6.
Rubinsztein DC, Leggo J, Barton DE, Ferguson-Smith MA. (1993). Site of (CCG) polymorphism
in the HD gene. Nat Genet. 5(3):214-5.
Thomas SM, Moreno RF, Tilzer L1. (1989). DNA extraction with organic solvents in gel barrier
tubes. Nucleic Acids Res. 17(13):5411.
Wittwer CT, Marshall BC, Reed GH, Cherry J1. (1993). Rapid cycle aJlele-specific amplification:
studies with the cystic fibrosis delta F50810cus. Clin Chern. 39(5):804-9.